SAP/SH2D1A Active Protein | SH2D1A active protein

SAP/SH2D1A Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120703

• Gene Names: SH2D1A; LYP; SAP; XLP; DSHP; EBVS; IMD5; XLPD; MTCP1; XLPD1; SAP/SH2D1A
• Purity: >94% as determined by SDS-PAGE
• Synonyms: SAP/SH2D1A; SAP/SH2D1A Protein; Human; Recombinant (His Tag); Human SH2D1A Protein (His Tag); DSHP Protein; EBVS Protein; IMD5 Protein; LYP Protein; MTCP1 Protein; SAP Protein; XLP Protein; XLPD Protein; XLPD1 Protein; SH2 domain containing 1A; SH2D1A active protein

• Host: E Coli
• Purity/Purification: >94% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.5, 20% glycerol. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Pro2-Lys97

• Species: Human
• Activity: 1. Measured by its ability to bind recombinant human SLAMF1 in a functional ELISA.; 2. Measured by its ability to bind recombinant human SLAMF6 in a functional ELISA.; 3. Measured by its ability to bind recombinant mouse SLAMF6 in a functional ELISA.; 4. Measured by its ability to bind recombinant mouse CD84 in a functional ELISA.
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human SH2D1A isoform 1 (NP_002342.1)(Pro 2-Lys 97) was expressed, with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for SH2D1A active protein

Background: SH₂ domain-containing protein 1A (SH2D1A/SAP) is a 128 amino acid protein, containing a single Src homology 2 (SH₂) domain, flanked by 5 amino acids at the N-terminus and 25 amino acids at the C-terminus. The absence of a catalytic domain and the presence of an SH₂ domain suggest that SH2D1A regulates one or more signal transduction pathways. SH2D1A interacts with signaling lymphocytic activation molecule (SLAM), which is a transmembrane protein expressed on the surface of activated T and B cells. SH2D1A (SAP) interacts via its SH₂ domain with a motif (TIYXXV) present in the cytoplasmic tail of the cell-surface receptors, including CD150/SLAM, CD84, CD229/Ly-9, and CD244/2B4. SH2D1A was expressed in EBV-carrying, tumor phenotype representative (type I), but not in EBV-carrying lymphoblastoid cell line (LCL)-like (type III) or EBV-negative Burkitt lymphoma (BL) lines. It has been supposed to be related to the X-linked lymphoproliferative disease which is also known as Duncan's disease or Purtilo syndrome.

Product Categories/Family for SH2D1A active protein

Adaptor Proteins

References

Morra M, et al. (2005) Defective B cell responses in the absence of SH2D1A. PNAS. 102 (13): 4819-23.Morra M, et al. (2001) Characterization of SH2D1A Missense Mutations Identified in X-linked Lymphoproliferative Disease Patients. The Journal of Biological Chemistry. 276: 36809-16.Hron JD, et al. (2004) SH2D1A Regulates T-dependent Humoral Autoimmunity. JEM. 200 (2): 261-6.

NCBI and Uniprot Product Information

• NCBI GI #: 4506923
• NCBI GeneID: 4068
• NCBI Accession #: NP_002342.1
• NCBI GenBank Nucleotide #: NM_002351.4
• UniProt Accession #: O60880
• NCBI Official Full Name: SH2 domain-containing protein 1A isoform 1
• NCBI Official Synonym Full Names: SH2 domain containing 1A
• NCBI Official Symbol: SH2D1A
• NCBI Official Synonym Symbols: LYP; SAP; XLP; DSHP; EBVS; IMD5; XLPD; MTCP1; XLPD1; SAP/SH2D1A
• NCBI Protein Information: SH2 domain-containing protein 1A
• UniProt Protein Name: SH2 domain-containing protein 1A
• Protein Family: SH2 domain-containing protein
• UniProt Gene Name: SH2D1A
• UniProt Synonym Gene Names: DSHP; SAP; SLAM-associated protein
• UniProt Entry Name: SH21A_HUMAN

NCBI Description

This gene encodes a protein that plays a major role in the bidirectional stimulation of T and B cells. This protein contains an SH2 domain and a short tail. It associates with the signaling lymphocyte-activation molecule, thereby acting as an inhibitor of this transmembrane protein by blocking the recruitment of the SH2-domain-containing signal-transduction molecule SHP-2 to its docking site. This protein can also bind to other related surface molecules that are expressed on activated T, B and NK cells, thereby modifying signal transduction pathways in these cells. Mutations in this gene cause lymphoproliferative syndrome X-linked type 1 or Duncan disease, a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus, with symptoms including severe mononucleosis and malignant lymphoma. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]