SH2 Domain-Containing Protein 1A Active Protein | SH2D1A active protein

Recombinant Human SH2 Domain-Containing Protein 1A Protein

Cat. Num. AAA2556679

• Gene Names: SH2D1A; LYP; SAP; XLP; DSHP; EBVS; IMD5; XLPD; MTCP1; XLPD1; SAP/SH2D1A
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: SH2 Domain-Containing Protein 1A; Recombinant Human SH2 Domain-Containing Protein 1A Protein; Duncan Disease SH2-Protein; Signaling Lymphocytic Activation Molecule-Associated Protein; SLAM-Associated Protein; T-Cell Signal Transduction Molecule SAP; SH2D1A; DSHP; SAP; SH2D1A active protein

• Host: E Coli
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Supplied as a 0.2 mum filtered solution of 20mM Tris, 150mM NaCl, 10% Glycerol, pH 7.5.
• Sequence Length: 128

• Species: Human
• Endotoxin: <1.0 EU per ug as determined by LAL test.
• Fragment: Met1-Pro128
• Fusion Tag: N-6His
• Activity: Measured by its ability to bind recombinant human SLAMF1 in a functional ELISA. 2. Measured by its ability to bind recombinant human SLAMF6 in a functional ELISA. 3. Measured by its ability to bind recombinant mouse SLAMF6 in a functional ELISA. 4. Measured by its ability to bind recombinant mouse CD84 in a functional ELISA.
• Preparation and Storage: Store at < -20 degree C, stable for 6 months after receipt.; Please minimize freeze-thaw cycles.

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Related Product Information for SH2D1A active protein

Protein Construction: Recombinant Human SH2 Domain-Containing Protein 1A is produced by our E Coli expression system and the target gene encoding Met1-Pro128 is expressed with a 6His tag at the N-terminus.

Background: SH2 Domain-Containing Protein 1A,(SH2D1A) contains one SH2 domain and a short tail and is localized in the cytoplasm. SH2D1A is expressed at a high level in the thymus and the lung, with lower expression levels in the spleen and the liver. SH2D1A acts as an inhibitor of the signaling lymphocyte activation molecule (SLAM) self-association. In addition, SH2D1A mediates interaction between FYN and SLAMF1. It is also thought to regulate the activity of the neurotrophin receptors NTRK1, NTRK2 and NTRK3.

NCBI and Uniprot Product Information

• NCBI GI #: 4506923
• NCBI GeneID: 4068
• NCBI Accession #: NP_002342.1
• NCBI GenBank Nucleotide #: NM_002351.4
• UniProt Accession #: O60880; O95383; O95384; O95385; O95386; Q6FGS6; Q9UNR0; A8MSW0
• Molecular Weight: Molecular Mass: 16.3kDa; Actual Protein Molecular Mass: 16kDa
• NCBI Official Full Name: SH2 domain-containing protein 1A isoform 1
• NCBI Official Synonym Full Names: SH2 domain containing 1A
• NCBI Official Symbol: SH2D1A
• NCBI Official Synonym Symbols: LYP; SAP; XLP; DSHP; EBVS; IMD5; XLPD; MTCP1; XLPD1; SAP/SH2D1A
• NCBI Protein Information: SH2 domain-containing protein 1A
• UniProt Protein Name: SH2 domain-containing protein 1A
• Protein Family: SH2 domain-containing protein
• UniProt Gene Name: SH2D1A
• UniProt Synonym Gene Names: DSHP; SAP; SLAM-associated protein

NCBI Description

This gene encodes a protein that plays a major role in the bidirectional stimulation of T and B cells. This protein contains an SH2 domain and a short tail. It associates with the signaling lymphocyte-activation molecule, thereby acting as an inhibitor of this transmembrane protein by blocking the recruitment of the SH2-domain-containing signal-transduction molecule SHP-2 to its docking site. This protein can also bind to other related surface molecules that are expressed on activated T, B and NK cells, thereby modifying signal transduction pathways in these cells. Mutations in this gene cause lymphoproliferative syndrome X-linked type 1 or Duncan disease, a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus, with symptoms including severe mononucleosis and malignant lymphoma. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Cytoplasmic adapter regulating receptors of the signaling lymphocytic activation molecule (SLAM) family such as SLAMF1, CD244, LY9, CD84, SLAMF6 and SLAMF7. In SLAM signaling seems to cooperate with SH2D1B/EAT-2. Initially it has been proposed that association with SLAMF1 prevents SLAMF1 binding to inhibitory effectors including INPP5D/SHIP1 and PTPN11/SHP-2 (PubMed:11806999). However, by simultaneous interactions, recruits FYN which subsequently phosphorylates and activates SLAMF1 (PubMed:12458214). Positively regulates CD244/2B4- and CD84-mediated natural killer (NK) cell functions. Can also promote CD48-, SLAMF6 -, LY9-, and SLAMF7-mediated NK cell activation. In the context of NK cell-mediated cytotoxicity enhances conjugate formation with target cells (). May also regulate the activity of the neurotrophin receptors NTRK1, NTRK2 and NTRK3.