SH2D1A recombinant protein

Recombinant Human SH2D1A Protein (His tag)

Cat. Num. AAA2545895

• Gene Names: SH2D1A; LYP; SAP; XLP; DSHP; EBVS; IMD5; XLPD; MTCP1; XLPD1; SAP/SH2D1A
• Purity: > 94 % as determined by SDS-PAGE
• Synonyms: SH2D1A; Recombinant Human SH2D1A Protein (His tag); RP5-1052M9.3; DSHP; EBVS; FLJ18687; FLJ92177; IMD5; LYP; MTCP1; SAP; SAP/SH2D1A; XLP; XLPD; SH2D1A recombinant protein

• Host: E Coli
• Purity/Purification: > 94 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.5, 20% glycerol
• Sequence Length: 128

• Application Notes: The recombinant human SH2D1A consisting of 139 amino acids and has a calculated molecular mass of 15.6 kDa.It migrates as an approximately 14 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for SH2D1A recombinant protein

Background: SH2 domain-containing protein 1A (SH2D1A / SAP) is a 128 amino acid protein, containing a single Src homology 2 (SH2) domain, flanked by 5 amino acids at the N-terminus and 25 amino acids at the C-terminus. The absence of a catalytic domain and the presence of an SH2 domain suggest that SH2D1A regulates one or more signal transduction pathways. SH2D1A interacts with signaling lymphocytic activation molecule (SLAM), which is a transmembrane protein expressed on the surface of activated T and B cells. SH2D1A (SAP) interacts via its SH2 domain with a motif (TIYXXV) present in the cytoplasmic tail of the cell-surface receptors, including CD150 / SLAM, CD84, CD229 / Ly-9, and CD244 / 2B4. SH2D1A was expressed in EBV-carrying, tumor phenotype representative (type I), but not in EBV-carrying lymphoblastoid cell line (LCL)-like (type III) or EBV-negative Burkitt lymphoma (BL) lines. It has been supposed to be related to the X-linked lymphoproliferative disease which is also known as Duncan's disease or Purtilo syndrome. 

Description: A DNA sequence encoding the human SH2D1A isoform 1 (NP_002342.1) (Pro 2-Lys 97) was expressed, with a polyhistidine tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 4506923
• NCBI GeneID: 4068
• NCBI Accession #: NP_002342.1
• NCBI GenBank Nucleotide #: NM_002351.4
• UniProt Accession #: O60880; O95383; O95384; O95385; O95386; Q6FGS6; Q9UNR0; A8MSW0
• Molecular Weight: 6,631 Da
• NCBI Official Full Name: SH2 domain-containing protein 1A isoform 1
• NCBI Official Synonym Full Names: SH2 domain containing 1A
• NCBI Official Symbol: SH2D1A
• NCBI Official Synonym Symbols: LYP; SAP; XLP; DSHP; EBVS; IMD5; XLPD; MTCP1; XLPD1; SAP/SH2D1A
• NCBI Protein Information: SH2 domain-containing protein 1A
• UniProt Protein Name: SH2 domain-containing protein 1A
• Protein Family: SH2 domain-containing protein
• UniProt Gene Name: SH2D1A
• UniProt Synonym Gene Names: DSHP; SAP; SLAM-associated protein

NCBI Description

This gene encodes a protein that plays a major role in the bidirectional stimulation of T and B cells. This protein contains an SH2 domain and a short tail. It associates with the signaling lymphocyte-activation molecule, thereby acting as an inhibitor of this transmembrane protein by blocking the recruitment of the SH2-domain-containing signal-transduction molecule SHP-2 to its docking site. This protein can also bind to other related surface molecules that are expressed on activated T, B and NK cells, thereby modifying signal transduction pathways in these cells. Mutations in this gene cause lymphoproliferative syndrome X-linked type 1 or Duncan disease, a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus, with symptoms including severe mononucleosis and malignant lymphoma. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

SH2D1A: Inhibitor of the SLAM self-association. Acts by blocking recruitment of the SH2-domain-containing signal-transduction molecule SHP-2 to a docking site in the SLAM cytoplasmic region. Mediates interaction between FYN and SLAMF1. May also regulate the activity of the neurotrophin receptors NTRK1, NTRK2 and NTRK3. Interacts with NTRK1, NTRK2 and NTRK3. Interacts with CD84, CD244, LY9, SLAMF1 and FYN. Expressed at a high level in thymus and lung, with a lower level of expression in spleen and liver. Expressed in peripheral blood leukocytes, including T lymphocytes. Tends to be expressed at lower levels in peripheral blood leukocytes in patients with rheumatoid arthritis. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Apoptosis

Chromosomal Location of Human Ortholog: Xq25

Cellular Component: cytoplasm; cytosol

Molecular Function: protein binding

Biological Process: cell-cell signaling; regulation of immune response

Disease: Lymphoproliferative Syndrome, X-linked, 1