Rantes Active Protein | CCL5 active protein

Recombinant Human Rantes (CCL5)

Cat. Num. AAA142035

• Gene Names: CCL5; SISd; eoCP; SCYA5; RANTES; TCP228; D17S136E; SIS-delta
• Purity: Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Synonyms: Rantes; Recombinant Human Rantes (CCL5); Rantes Human; Rantes Human Recombinant (CCL5); Small inducible cytokine A5; CCL5; T-cell-specific RANTES protein; SIS-delta; T cell- specific protein P228; TCP228; chemokine (C-C motif) ligand 5; SISd; SCYA5; RANTES; D17S136E; MGC17164; CCL5 active protein

• Host: E Coli
• Purity/Purification: Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 um filtered concentrated solution in 50mM NaAc, pH 5.0, 100mM NaCl.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: SPYSSDTTPC CFAYIARPLP RAHIKEYFYT SGKCSNPAVV FVTRKNRQVC ANPEKKWVRE YINSLEMS
• Sequence Length: 91

• Solubility: It is recommended to reconstitute the lyophilized Rantes in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: The Rantes activity is determined by the chemoattract of human blood monocytes at a concentration between 1-10ng/ml corresponding to a Specific Activity of 100,000-1,000,000IU/mg.
• Preparation and Storage: Lyophilized Rantes although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution Rantes should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Related Product Information for CCL5 active protein

Description: Rantes Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 68 amino acids and having a molecular mass of 7809.2 Dalton. The Rantes is purified by proprietary chromatographic techniques.

Introduction: Regulated upon Activation, Normal T-cell Expressed, and Secreted or RANTES is an 8 kDa protein classified as a chemotactic cytokine or chemokine. Rantes has recently been renamed CCL5. RANTES is chemotactic for T cells, eosinophils and basophils and plays an active role in recruiting leukocytes into inflammatory sites. With the help of particular cytokines (i.e. IL-2 and IFN-g) that are released by T cells, RANTES also induces the proliferation and activation of certain natural killer (NK) cells to form CHAK (CC-Chemokine-activated killer) cells. Rantes is also an HIV-suppressive factor released from CD8+ T cells. The Rantes chemokine has been localized to chromosome 17 in humans.

Product Categories/Family for CCL5 active protein

Chemokines; Rantes (CCL5)

NCBI and Uniprot Product Information

• NCBI GI #: 22538814
• NCBI GeneID: 6352
• NCBI Accession #: NP_002976.2
• NCBI GenBank Nucleotide #: NM_002985.2
• UniProt Accession #: P13501; O43646; Q0QVW8; Q4ZGJ1; Q9NYA2; Q9UBG2; Q9UC99
• Molecular Weight: 9,990 Da
• NCBI Official Full Name: C-C motif chemokine 5 isoform 1
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 5
• NCBI Official Symbol: CCL5
• NCBI Official Synonym Symbols: SISd; eoCP; SCYA5; RANTES; TCP228; D17S136E; SIS-delta
• NCBI Protein Information: C-C motif chemokine 5; T-cell specific protein p288; beta-chemokine RANTES; eosinophil chemotactic cytokine; regulated upon activation, normally T-expressed, and presumably secreted; small inducible cytokine subfamily A (Cys-Cys), member 5
• UniProt Protein Name: C-C motif chemokine 5
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL5
• UniProt Synonym Gene Names: D17S136E; SCYA5; TCP228
• UniProt Entry Name: CCL5_HUMAN

NCBI Description

This gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Jul 2013]

Uniprot Description

CCL5: Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T- cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils. By mitogens. T-cell and macrophage specific. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted; Secreted, signal peptide; Chemokine; Motility/polarity/chemotaxis; Cell adhesion

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: extracellular space; cytoplasm; extracellular region

Molecular Function: CCR4 chemokine receptor binding; protein binding; CCR1 chemokine receptor binding; protein homodimerization activity; protein self-association; chemokine receptor binding; chemokine activity; chemokine receptor antagonist activity; receptor signaling protein tyrosine kinase activator activity; phosphoinositide phospholipase C activity; CCR5 chemokine receptor binding; phospholipase activator activity; chemoattractant activity; protein kinase activity

Biological Process: regulation of chronic inflammatory response; positive regulation of cell adhesion; exocytosis; positive regulation of smooth muscle cell proliferation; response to toxin; positive regulation of JAK-STAT cascade; chemotaxis; positive regulation of smooth muscle cell migration; positive regulation of cell-cell adhesion mediated by integrin; protein amino acid phosphorylation; positive regulation of homotypic cell-cell adhesion; positive regulation of translational initiation; monocyte chemotaxis; leukocyte adhesion; cell-cell signaling; positive chemotaxis; calcium ion transport; positive regulation of innate immune response; protein kinase B signaling cascade; lipopolysaccharide-mediated signaling pathway; dendritic cell chemotaxis; positive regulation of T cell proliferation; inflammatory response; protein tetramerization; phospholipase D activation; positive regulation of viral genome replication; neutrophil activation; negative regulation of G-protein coupled receptor protein signaling pathway; response to virus; MAPKKK cascade; positive regulation of cellular biosynthetic process; macrophage chemotaxis; cellular calcium ion homeostasis; positive regulation of phosphoinositide 3-kinase cascade; G-protein coupled receptor protein signaling pathway; cellular protein complex assembly; negative regulation of viral genome replication; positive regulation of tyrosine phosphorylation of STAT protein; eosinophil chemotaxis; positive regulation of calcium ion transport; regulation of insulin secretion; positive regulation of epithelial cell proliferation; positive regulation of phosphorylation; positive regulation of cell migration; regulation of T cell activation