SHP-1 Active Protein | PTPN6 active protein

Human Recombinant SHP-1

Cat. Num. AAA844577

• Gene Names: PTPN6; HCP; HCPH; SHP1; SHP-1; HPTP1C; PTP-1C; SHP-1L; SH-PTP1
• Applications: SDS-Page
• Purity: >95% by SDS-PAGE
• Synonyms: SHP-1; Human Recombinant SHP-1; Protein tyrosine phosphatase; non-receptor type 6 isoform 1; PTPN6; HCP; HCPH; HPTP1C; PTP-1C; SH-PTP1; SHP-1L; SHP1.; PTPN6 active protein

• Purity/Purification: >95% by SDS-PAGE
• Form/Format: 1 mg/ml solution in 25 mM Tris-HCl buffer (pH 7.5) containing 1 mM DTT, 2 mM beta-mercaptoethanol, 1 mM EDTA, 20% glycerol.; Appearance: Liquid
• Concentration: 1 mg/ml (varies by lot)
• Sequence: MGFWEEFESL QKQEVKNLHQ RLEGQRPENK GKNRYKNILP FDHSRVILQG RDSNIPGSDY INANYIKNQL LGPDENAKTY IASQGCLEAT VNDFWQMAWQ ENSRVIVMTT REVEKGRNKC VPYWPEVGMQ RAYGPYSVTN CGEHDTTEYK LRTLQVSPLD NGDLIREIWH YQYLSWPDHG VPSEPGGVLS FLDQINQRQE SLPHAGPIIV HCSAGIGRTG TIIVIDMLME NISTKGLDCD IDIQKTIQMV RAQRSGMVQT EAQYKFIYVA IAQFIETTKK KLEVLQSQKG QESEYGNITY
• Sequence Length: 595

• Applicable Applications for PTPN6 active protein: SDS-PAGE
• Source: E Coli
• Endotoxin Level: < 1.0 EU per 1 ug of protein (determined by LAL method)
• Biological Activity: >5,000 units/mg of SHP-1.
• Unit Definition: One unit will hydrolyze 1 nanomole of p-nitrophenylphosphatate (pNPP) per minute at pH 7.4 at 37 degree C using 10 mM of substrate.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C to -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Related Product Information for PTPN6 active protein

Background: The protein coding region of the catalytic domain of SHP-1 (amino acids 243-541) was cloned into an E. coli expression vector. The catalytic domain of SHP-1 was overexpressed as insoluble protein aggregates (inclusion bodies). The recombinant SHP-1 protein was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer. Additional amino acid (Met) is attached at N-terminus.

Product Categories/Family for PTPN6 active protein

Proteins and Enzymes; Proteins and Enzymes (A-Z)  Diabetes; Obesity & Metabolic Syndrome; Signaling Pathways in Diabetes and Obesity; AMPK; Insulin and Leptin Signaling 

NCBI and Uniprot Product Information

• NCBI GI #: 18104989
• NCBI GeneID: 5777
• NCBI Accession #: NP_002822.2
• NCBI GenBank Nucleotide #: NM_002831.5
• UniProt Accession #: P29350; Q969V8; Q9UK67; A8K306; G3V0F8
• Molecular Weight: 34.3 kDa (300 aa) confirmed by MALDI-TOF
• NCBI Official Full Name: tyrosine-protein phosphatase non-receptor type 6 isoform 1
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, non-receptor type 6
• NCBI Official Symbol: PTPN6
• NCBI Official Synonym Symbols: HCP; HCPH; SHP1; SHP-1; HPTP1C; PTP-1C; SHP-1L; SH-PTP1
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type 6
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type 6
• Protein Family: Tyrosine-protein phosphatase non-receptor
• UniProt Gene Name: PTPN6
• UniProt Synonym Gene Names: HCP; PTP1C; PTP-1C
• UniProt Entry Name: PTN6_HUMAN

NCBI Description

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling. Multiple alternatively spliced variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

SHP-1: an SH2-containing ubiquitously expressed tyrosine-specific protein phosphatase. Plays a key role in hematopoiesis. Directly links growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Three alternatively spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; EC 3.1.3.48; Protein phosphatase, tyrosine (non-receptor)

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: membrane; cytoplasm; nucleolus; alpha-beta T cell receptor complex; intercellular junction; nucleus; cytosol

Molecular Function: protein binding; phosphotyrosine binding; transmembrane receptor protein tyrosine phosphatase activity; protein tyrosine phosphatase activity; SH2 domain binding; SH3 domain binding; protein kinase binding

Biological Process: negative regulation of MAP kinase activity; peptidyl-tyrosine phosphorylation; apoptosis; protein amino acid dephosphorylation; negative regulation of humoral immune response mediated by circulating immunoglobulin; negative regulation of cell proliferation; B cell receptor signaling pathway; negative regulation of T cell proliferation; negative regulation of B cell receptor signaling pathway; positive regulation of cell proliferation; abortive mitotic cell cycle; cell differentiation; negative regulation of peptidyl-tyrosine phosphorylation; natural killer cell mediated cytotoxicity; cytokine and chemokine mediated signaling pathway; negative regulation of T cell receptor signaling pathway; positive regulation of cell adhesion mediated by integrin; regulation of release of sequestered calcium ion into cytosol; positive regulation of phosphoinositide 3-kinase cascade; G-protein coupled receptor protein signaling pathway; cell proliferation; T cell costimulation; regulation of B cell differentiation; platelet formation; blood coagulation; leukocyte migration