Rabbit anti-Human PLA2G4B Polyclonal Antibody | anti-PLA2G4B antibody

PLA2G4B, ID (PLA2G4B, Cytosolic phospholipase A2 beta, Phospholipase A2 group IVB) (FITC)

Cat. Num. AAA6334556

• Gene Names: PLA2G4B; HsT16992; cPLA2-beta
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PLA2G4B; Polyclonal Antibody; ID (PLA2G4B; Cytosolic phospholipase A2 beta; Phospholipase A2 group IVB) (FITC); Phospholipase A2 group IVB; anti-PLA2G4B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-PLA2G4B antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PLA2G4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acid 400-430 from the Central region of human PLA2G4B.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PLA2G4B Antibody (Center) in K562 cell line lysates (35ug/lane). PLA2G4B (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(PLA2G4B Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-PLA2G4B antibody

PLA2G4B transcribes naturally-occurring mRNAs that are co-transcribed products of the neighboring JMJD7 and PLA2G4B genes. Incompletely processed read-through transcripts from these two loci are abundantly expressed in most tissues, but the function of the predicted protein product has not yet been determined.

Product Categories/Family for anti-PLA2G4B antibody

Antibodies; Enzymes; Phospholipase

References

Ghosh, M., et al. J. Biol. Chem. 281(24):16615-16624(2006). Tao, R., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 137B (1), 56-58 (2005). Pope, S.N., et al. J. Steroid Biochem. Mol. Biol. 94 (1-3), 203-208 (2005).

NCBI and Uniprot Product Information

• NCBI GI #: 167860120
• NCBI GeneID: 100137049
• NCBI Accession #: NP_001108105.1
• NCBI GenBank Nucleotide #: NM_001114633.1
• UniProt Accession #: P0C869; O95712; Q19KD5; Q19KD6; Q59GF9; Q8TB10; Q9UKV7; B4DRT9
• Molecular Weight: 114,121 Da
• NCBI Official Full Name: cytosolic phospholipase A2 beta
• NCBI Official Synonym Full Names: phospholipase A2, group IVB (cytosolic)
• NCBI Official Symbol: PLA2G4B
• NCBI Official Synonym Symbols: HsT16992; cPLA2-beta
• NCBI Protein Information: cytosolic phospholipase A2 beta
• UniProt Protein Name: Cytosolic phospholipase A2 beta
• Protein Family: Cytosolic phospholipase
• UniProt Gene Name: PLA2G4B
• UniProt Synonym Gene Names: cPLA2-beta
• UniProt Entry Name: PA24B_HUMAN

NCBI Description

This gene encodes a member of the cytosolic phospholipase A2 protein family. Phospholipase A2 enzymes hydrolyze the sn-2 bond of phospholipids, releasing lysophospholipids and fatty acids. This enzyme may be associated with mitochondria and early endosomes. Most tissues also express read-through transcripts from the upstream gene into this gene, some of which may encode fusion proteins combining the N-terminus of the upstream gene including its JmjC domain with the almost complete coding region of this gene, including the C2 and cytoplasmic phospholipase A2 domains. [provided by RefSeq, Jul 2008]

Uniprot Description

PLA2G4B: a calcium-dependent phospholipase A2 that catalyzes the release of arachidonic acid from membrane phospholipids. Selectively hydrolyzes glycerophospholipids in the sn-2 position with a preference for arachidonoyl phospholipids. Has a much weaker activity than PLA2G4A. Not inhibited by pyrrolidine-2 and Wyeth-1. The N-terminal C2 domain associates with lipid membranes and mediates its regulation by presenting the active site to its substrate in response to elevations of cytosolic Ca2+. Four isoforms of the human protein are produced by alternative splicing. Isoform 3 translocates to membrane vesicles in a calcium-dependent fashion and has activity against palmitoyl-arachidonyl-phosphatidylethanolamine and low level lysophospholipase activity but no activity against phosphatidylcholine.

Protein type: Phospholipase; EC 3.1.1.4; Lipid Metabolism - arachidonic acid

Chromosomal Location of Human Ortholog: 15q11.2-q21.3

Cellular Component: early endosome membrane; mitochondrial inner membrane; extracellular region; cytosol

Molecular Function: calcium-dependent phospholipid binding; calcium-dependent phospholipase A2 activity; calcium ion binding; lysophospholipase activity

Biological Process: unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; unfolded protein response; glycerophospholipid catabolic process; phospholipid metabolic process; glycerophospholipid biosynthetic process; calcium-mediated signaling; arachidonic acid metabolic process; phosphatidic acid biosynthetic process; parturition; inflammatory response