MCP4 Active Protein | Mcpt4 active protein

MCP4, Recombinant, Human (Macrophage/Monocyte Chemotactic Protein 4)

Cat. Num. AAA650349

• Gene Names: Mcpt4; Mcp4; Mcp-4; MMCP-4; MMCP-4A; MMCP-4B
• Purity: Highly Purified; 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
• Synonyms: MCP4; Recombinant; Human (Macrophage/Monocyte Chemotactic Protein 4); Mcpt4 active protein

• Host: E Coli
• Purity/Purification: Highly Purified; 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
• Form/Format: Supplied as a lyophilized powder. No additives.
• Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Gln-Pro-Asp-Ala-Leu.

• Biological Activity: MCP-4 is fully biologically active when compared to standard. The specific activity as determined by the ability of MCP-4 to chemoattaract human monocytes at 10-100ng/ml.
• Dimers and Aggregates: 1% as determined by silver-stained SDS-PAGE gel analysis.
• Endotoxin Level: 0.1ng/ug (IEU/ug) of Human Monocyte Chemotactic Protein-4.
• Protein Content: Protein quantitation was carried out by two independent methods:; 1. UV spectroscopy at 280nm.; 2. Analysis by RP-HPLC, using a calibrated solution of Human MCP-4 as a Reference Standard.
• Reconstitution: Reconstitute the lyophilized MCP-4 in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for Mcpt4 active protein

Recombinant Human MCP-4 is a non-glycosylated, Polypeptide chain containing 74 amino acids and having a molecular mass of 8510 Dalton.

Product Categories/Family for Mcpt4 active protein

Growth Factors; Cytokines; Growth Factors-MCP

NCBI and Uniprot Product Information

• NCBI GI #: 114205406
• NCBI GeneID: 17227
• NCBI Accession #: NP_034909.2
• NCBI GenBank Nucleotide #: NM_010779.2
• UniProt Accession #: P21812; Q9EPQ9; Q9EQT2
• Molecular Weight: 27,204 Da
• NCBI Official Full Name: mast cell protease 4
• NCBI Official Synonym Full Names: mast cell protease 4
• NCBI Official Symbol: Mcpt4
• NCBI Official Synonym Symbols: Mcp4; Mcp-4; MMCP-4; MMCP-4A; MMCP-4B
• NCBI Protein Information: mast cell protease 4; MSMCP; myonase; serosal mast cell protease
• UniProt Protein Name: Mast cell protease 4
• Protein Family: Mast cell protease
• UniProt Gene Name: Mcpt4
• UniProt Synonym Gene Names: mMCP-4
• UniProt Entry Name: MCPT4_MOUSE

Uniprot Description

Function: Has chymotrypsin-like activity. Hydrolyzes the amide bonds of synthetic substrates having Tyr and Phe residues at the P1 position. Preferentially hydrolyzes the 'Tyr-4-|-Ile-5' bond of angiotensin I and the 'Phe-20-|-Ala-21' bond of amyloid beta-protein, and is less active towards the 'Phe-8-|-His-9' bond of angiotensin I and the 'Phe-4-|-Ala-5' and 'Tyr-10-|-Glu-11' bonds of amyloid beta-protein. Involved in thrombin regulation and fibronectin processing. Ref.4 Ref.7

Enzyme regulation: Completely inhibited by serine protease inhibitors such as chymostatin, diisopropylfluorophosphate and phenylmethylsulfonyl fluoride, but not by p-tosyl-L-phenylalanine chloromethyl ketone, p-tosyl-L-lysine chloromethyl ketone, pepstatin, E-64, EDTA or o-phenanthroline. Also inhibited by lima bean trypsin inhibitor, soy bean trypsin inhibitor and human plasma alpha1-antichymotrysin.

Subunit structure: Monomer. Ref.4

Tissue specificity: Submucosal mast cells. In femoral muscle, detected in myocytes but not in mast cells. Ref.4

Disruption phenotype: Mice display an impaired ability to inactivate thrombin or degrade fibronectin in peritoneal cells. Ref.7

Sequence similarities: Belongs to the peptidase S1 family. Granzyme subfamily.Contains 1 peptidase S1 domain.

Biophysicochemical propertiespH dependence:Optimum pH is 9 at high salt concentrations.

Mass spectrometry: Molecular mass is 25187 Da from positions 21 - 246. Determined by MALDI. Ref.4

Sequence caution: The sequence BAB18732.1 differs from that shown. Reason: Erroneous initiation.