Mouse Mcpt4 (Mast cell protease 4) ELISA Kit | Mcpt4 elisa kit

Mouse Mcpt4 (Mast cell protease 4) ELISA Kit

Cat. Num. AAA7606603

• Gene Names: Mcpt4; Mcp4; Mcp-4; MMCP-4; MMCP-4A; MMCP-4B
• Reactivity: Mouse
• Synonyms: Mcpt4 (Mast cell protease 4); Mouse Mcpt4 (Mast cell protease 4) ELISA Kit; Mcpt4; mast cell protease 4; MSMCP; Serosal mast cell protease; Mcpt4 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mcpt4. No significant cross-reactivity or interference between Mcpt4 and analogues was observed.
• Sequence Length: 246

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 46.875-3000pg/ml
• Sensitivity: 28.125pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Mcpt4 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 114205406
• NCBI GeneID: 17227
• NCBI Accession #: NP_034909.2
• NCBI GenBank Nucleotide #: NM_010779.2
• UniProt Accession #: P21812; Q9EPQ9; Q9EQT2
• Molecular Weight: 27,204 Da
• NCBI Official Full Name: mast cell protease 4
• NCBI Official Synonym Full Names: mast cell protease 4
• NCBI Official Symbol: Mcpt4
• NCBI Official Synonym Symbols: Mcp4; Mcp-4; MMCP-4; MMCP-4A; MMCP-4B
• NCBI Protein Information: mast cell protease 4
• UniProt Protein Name: Mast cell protease 4
• Protein Family: Mast cell protease
• UniProt Gene Name: Mcpt4
• UniProt Synonym Gene Names: mMCP-4

Uniprot Description

Mcpt4: Has chymotrypsin-like activity. Hydrolyzes the amide bonds of synthetic substrates having Tyr and Phe residues at the P1 position. Preferentially hydrolyzes the 'Tyr-4-|-Ile-5' bond of angiotensin I and the 'Phe-20-|-Ala-21' bond of amyloid beta- protein, and is less active towards the 'Phe-8-|-His-9' bond of angiotensin I and the 'Phe-4-|-Ala-5' and 'Tyr-10-|-Glu-11' bonds of amyloid beta-protein. Involved in thrombin regulation and fibronectin processing. Belongs to the peptidase S1 family. Granzyme subfamily.

Protein type: EC 3.4.21.-; Protease

Chromosomal Location of Human Ortholog: 14 C3|14 28.19 cM

Cellular Component: intracellular

Molecular Function: peptidase activity; serine-type endopeptidase activity

Biological Process: protein processing; proteolysis; regulation of angiotensin levels in blood