LGALS3 active protein

LGALS3, 1-264aa, Mouse, His tag, E Coli (Bioactivity Validated)

Cat. Num. AAA206266

• Gene Names: Lgals3; GBP; L-34; gal3; Mac-2
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: LGALS3; 1-264aa; Mouse; His tag; E Coli (Bioactivity Validated); Lectin galactose binding soluble 3; Lectin; galactose binding; soluble 3; CBP35; GAL3; GALBP; GALIG; LGALS2; MAC2; LGALS3 active protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 50% glycerol, 1mM DTT, 2mM EDTA
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMADSFSL NDALAGSGNP NPQGYPGAWG NQPGAGGYPG AAYPGAYPGQ APPGAYPGQA PPGAYPGQAP PSAYPGPTAP GAYPGPTAPG AYPGSTAPGA FPGQPGAPGA YPSAPGGYPA AGPYGVPAGP LTVPYDLPLP GGVMPRMLIT IMGTVKPNAN RIVLDFRRGN DVAFHFNPRF NENNRRVIVC NTKQDNNWGK EERQSAFPFE SGKPFKIQVL VEADHFKVAV NDAHLLQYNH RMKNLREISQ LGISGDITLT SANHAMI
• Sequence Length: 264

• Applicable Applications for LGALS3 active protein: SDS-PAGE
• Antigen Species: Mouse
• Tag Information: His-tag
• Biological Activity: The ED50 for this effect is equal or higher than 25 ug/ml. Measured by its ability to agglutinate human red blood cells.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for LGALS3 active protein

LGALS3, also known as galectin 3, is a member of the family of animal lectins, which selectively binds beta-galactoside residues. This protein is secreted from cells by ectocytosis, which is independent of the classical secretory pathway through the endoplasmic reticulum/Golgi network. LGALS3 has been associated with the inhibition of apoptosis and the progression of cancer. It is normally distributed in epithelia of many organs, in various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins. Recombinant mouse LGALS3 protein, used to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for LGALS3 active protein

Cancer

References

Barondes SH., et al. (1994) J Biol Chem. 269 (33):20807-10.; Kadrofske MM., et al. (1998) Arch Biochem Biophys. 349 (1):7-20.

NCBI and Uniprot Product Information

• NCBI GI #: 33859580
• NCBI GeneID: 16854
• NCBI Accession #: NP_034835
• NCBI GenBank Nucleotide #: NM_010705.3
• UniProt Accession #: P16110
• Molecular Weight: 29.8 kDa (287aa) confirmed by MALDI-TOF
• NCBI Official Full Name: galectin-3
• NCBI Official Synonym Full Names: lectin, galactose binding, soluble 3
• NCBI Official Symbol: Lgals3
• NCBI Official Synonym Symbols: GBP; L-34; gal3; Mac-2
• NCBI Protein Information: galectin-3
• UniProt Protein Name: Galectin-3
• UniProt Gene Name: Lgals3
• UniProt Synonym Gene Names: Gal-3; CBP 35

Uniprot Description

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells ().