Rabbit anti-Human TRIP13 Polyclonal Antibody | anti-TRIP13 antibody

TRIP13, NT (Thyroid Receptor-interacting Protein 13, Thyroid Hormone Receptor Interactor 13, TR-interacting Protein 13, TRIP-13, Human Papillomavirus Type 16 E1 Protein-binding Protein, HPV16 E1 Protein-binding Protein, 16E1-BP) (MaxLight 490)

Cat. Num. AAA6502585

• Gene Names: TRIP13; MVA3; 16E1BP
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: TRIP13; Polyclonal Antibody; NT (Thyroid Receptor-interacting Protein 13; Thyroid Hormone Receptor Interactor 13; TR-interacting Protein 13; TRIP-13; Human Papillomavirus Type 16 E1 Protein-binding Protein; HPV16 E1 Protein-binding Protein; 16E1-BP) (MaxLight 490); anti-TRIP13 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TRIP13.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-TRIP13 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human TRIP13 (KLH).
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of TRIP13 Antibody (N-term) in K562, Hela, 293 cell line lysates (35ug/lane). TRIP13 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with NRP1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(TRIP13 Antibody (N-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Western blot analysis of TRIP13 (arrow) using rabbit polyclonal TRIP13 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TRIP13 gene.)

Product Categories/Family for anti-TRIP13 antibody

Antibodies; Binding Proteins

References

1. Rush, J., Nat. Biotechnol. 23(1), 94-101 (2005).

NCBI and Uniprot Product Information

• NCBI GI #: 11321607
• NCBI GeneID: 9319
• NCBI Accession #: NP_004228
• NCBI GenBank Nucleotide #: NM_004237
• UniProt Accession #: Q15645
• Molecular Weight: 49kDa
• NCBI Official Full Name: pachytene checkpoint protein 2 homolog isoform 1
• NCBI Official Synonym Full Names: thyroid hormone receptor interactor 13
• NCBI Official Symbol: TRIP13
• NCBI Official Synonym Symbols: MVA3; 16E1BP
• NCBI Protein Information: pachytene checkpoint protein 2 homolog
• UniProt Protein Name: Pachytene checkpoint protein 2 homolog
• UniProt Gene Name: TRIP13
• UniProt Synonym Gene Names: PCH2; 16E1-BP; HPV16 E1 protein-binding protein; TR-interacting protein 13; TRIP-13
• UniProt Entry Name: PCH2_HUMAN

NCBI Description

This gene encodes a protein that interacts with thyroid hormone receptors, also known as hormone-dependent transcription factors. The gene product interacts specifically with the ligand binding domain. This gene is one of several that may play a role in early-stage non-small cell lung cancer. [provided by RefSeq, Oct 2009]

Uniprot Description

TRIP13: Plays a key role in chromosome recombination and chromosome structure development during meiosis. Required at early steps in meiotic recombination that leads to non-crossovers pathways. Also needed for efficient completion of homologous synapsis by influencing crossover distribution along the chromosomes affecting both crossovers and non-crossovers pathways. Also required for development of higher-order chromosome structures and is needed for synaptonemal-complex formation. In males, required for efficient synapsis of the sex chromosomes and for sex body formation. Promotes early steps of the DNA double- strand breaks (DSBs) repair process upstream of the assembly of RAD51 complexes. Required for depletion of HORMAD1 and HORMAD2 from synapsed chromosomes. Belongs to the AAA ATPase family. PCH2 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 5p15.33

Cellular Component: male germ cell nucleus; nucleus

Molecular Function: identical protein binding; protein binding; transcription cofactor activity; ATP binding

Biological Process: oogenesis; transcription from RNA polymerase II promoter; synaptonemal complex assembly; male meiosis I; meiotic recombination; double-strand break repair; spermatogenesis; oocyte maturation; spermatid development; female meiosis I