Western Blot (WB)
(The Recombinant Human LDHB (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human LDHB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Recombinant LDHB protein Lane 2. : A549 cell lysate Lane 3. : 293T cell lysate Lane 4. : K562 cell lysate Lane 5. : Jurkat cell lysate)
Mouse anti-Human LDHB Monoclonal Antibody | anti-LDHB antibody
LDHB antibody
Gene Names
LDHB; LDH-B; LDH-H; LDHBD; TRG-5; HEL-S-281
Reactivity
Human
Applications
ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
Purity
By protein-A affinity chromatography
Synonyms
LDHB; Monoclonal Antibody; LDHB antibody; L-lactate dehydrogenase B chain; LDH-H; TRG-5.; LDH-B; LDH heart subunit; Renal carcinoma antigen NY-REN-46; anti-LDHB antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
AT14D7
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
334
Applicable Applications for anti-LDHB antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Antigen Species
Human
Immunogen
Recombinant human LDHB (1-334aa) purified from E. coli
Clone Note
Anti-human LDHB mAb, clone AT14D7, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human LDHB protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The Recombinant Human LDHB (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human LDHB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Recombinant LDHB protein Lane 2. : A549 cell lysate Lane 3. : 293T cell lysate Lane 4. : K562 cell lysate Lane 5. : Jurkat cell lysate)
Western Blot (WB)
(The Recombinant Human LDHB (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human LDHB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Recombinant LDHB protein Lane 2. : A549 cell lysate Lane 3. : 293T cell lysate Lane 4. : K562 cell lysate Lane 5. : Jurkat cell lysate)
Immunofluorescence (IF)
(ICC/IF analysis of LDHB in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human LDHB antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Immunofluorescence (IF)
(ICC/IF analysis of LDHB in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human LDHB antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Related Product Information for anti-LDHB antibody
LDHB, also known as L-lactate dehydrogenase B chain, is a member of the lactate dehydrogenase family. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As this protein can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD). The LDH family consists of three members, LDH-A, LDH-B and LDH-C. LDHs function as powerful markers for germ cell tumors.
Product Categories/Family for anti-LDHB antibody
References
Read JA., et al. (2001) Proteins. 43 (2): 175-185. ; Butt AA., et al. (2002) AIDS. 12 (7): 317-321.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
36,638 Da
NCBI Official Full Name
L-lactate dehydrogenase B chain isoform LDHB
NCBI Official Synonym Full Names
lactate dehydrogenase B
NCBI Official Symbol
LDHB
NCBI Official Synonym Symbols
LDH-B; LDH-H; LDHBD; TRG-5; HEL-S-281
NCBI Protein Information
L-lactate dehydrogenase B chain
UniProt Protein Name
L-lactate dehydrogenase B chain
Protein Family
UniProt Gene Name
LDHB
UniProt Synonym Gene Names
LDH-B; LDH-H
NCBI Description
This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes X, 5 and 13. [provided by RefSeq, Feb 2016]
Research Articles on LDHB
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Product Notes
The LDHB ldhb (Catalog #AAA200353) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The LDHB antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LDHB can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). Researchers should empirically determine the suitability of the LDHB ldhb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LDHB, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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