Interferon alpha 1b Active Protein | IFNA1 active protein
Interferon alpha 1b, Recombinant, Human (IFNa1b)
Gene Names
IFNA1; IFL; IFN; IFNA@; IFNA13; IFN-ALPHA; IFN-alphaD
Purity
Highly Purified98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Synonyms
Interferon alpha 1b; Recombinant; Human (IFNa1b); IFNA1 active protein
Host
E Coli
Purity/Purification
Highly Purified
98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Form/Format
Supplied as a powder lyophilized from a solution (1mg/ml) containing HSA and sodium chloride.
Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Cys-Asp-Leu-Pro-Glu.
Biological Activity
Interferon-alpha 1b is fully biologically active when compared to WHO standard. The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 1 x 10e8 IU/ mg.
Dimers and Aggregates
1% as determined by silver-stained SDS-PAGE gel analysis.
Endotoxin Level
0.1ng/ug (IEU/ug)
Protein Content
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm.
2. Analysis by RP-HPLC, using a standard solution of IFN-alpha 1b as a Reference Standard.
1. UV spectroscopy at 280nm.
2. Analysis by RP-HPLC, using a standard solution of IFN-alpha 1b as a Reference Standard.
Reconstitution
Reconstitute the lyophilized IFN alpha-1b in sterile dH2O to not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Preparation and Storage
Can be stored at +4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
Related Product Information for IFNA1 active protein
At least 23 different variants of IFN-alpha are known. The individual proteins have molecular masses between 19-26kD and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end. Interferons (IFNs) appear early after viral infection locally and systematically to limit spread of viral infection. They also affect cell differentiation, growth, surface antigen expression and immunoregulation. There are three naturally occurring interferons: alpha, beta and gamma. Interferon alpha (IFN-alpha, IFNa) is also known as leukocyte interferon. B-lymphocytes are the predominant cellular producers of INF-alpha. IFN-alpha is derived from lymphoblastic tissue. IFN-alpha comprises a family of related, homologous proteins, each exhibiting a unique activity profile. The activities of the different IFN-alpha species on viruses can vary twenty fold or more. INF-alpha has a number of therapeutic applications in the treatment of various human cancers and diseases of viral origin.
Product Categories/Family for IFNA1 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19392D
NCBI Official Full Name
interferon alpha-1/13
NCBI Official Synonym Full Names
interferon, alpha 1
NCBI Official Symbol
IFNA1
NCBI Official Synonym Symbols
IFL; IFN; IFNA@; IFNA13; IFN-ALPHA; IFN-alphaD
NCBI Protein Information
interferon alpha-1/13; leIF D; IFN-alpha 1b; IFN-alpha-1/13; interferon-alpha1; interferon alpha-D; interferon alpha 1b
UniProt Protein Name
Interferon alpha-1/13
Protein Family
UniProt Gene Name
IFNA1
UniProt Synonym Gene Names
IFN-alpha-1/13; LeIF D
UniProt Entry Name
IFNA1_HUMAN
NCBI Description
The protein encoded by this gene is produced by macrophages and has antiviral activity. This gene is intronless and the encoded protein is secreted. [provided by RefSeq, Sep 2011]
Uniprot Description
Function: Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. Ref.15
Subcellular location: Secreted.
Polymorphism: Two forms exist; alpha-1a (shown here) and alpha-1b.
Miscellaneous: Interferons alpha-1 and alpha-13 have identical protein sequences.
Sequence similarities: Belongs to the alpha/beta interferon family.
Research Articles on IFNA1
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Product Notes
The IFNA1 ifna1 (Catalog #AAA650448) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: The sequence of the first five N-terminal amino acids was determined and was found to be Cys-Asp-Le u-Pro-Glu. It is sometimes possible for the material contained within the vial of "Interferon alpha 1b, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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