Insulin-Like Growth Factor II (rHuIGF-II) Active Protein | IGF2 active protein

Recombinant Human Insulin-Like Growth Factor II(rHuIGF-II)

Cat. Num. AAA197012

• Gene Names: IGF2; IGF-II; PP9974; C11orf43; FLJ22066; FLJ44734
• Purity: Greater than 97% by RP-HPLC, anion-exchange chromatography; reducing and non-reducing SDS-PAGE, silver stain.; Purification: Chromatographic
• Synonyms: Insulin-Like Growth Factor II (rHuIGF-II); Recombinant Human Insulin-Like Growth Factor II(rHuIGF-II); Recombinant Human Insulin-Like Growth Factor II (rHuIGF-II); IGF2 active protein

• Host: E Coli
• Specificity: Recombinant Human Insulin-Like Growth Factor II (rHuIGF-II)
• Purity/Purification: Greater than 97% by RP-HPLC, anion-exchange chromatography; reducing and non-reducing SDS-PAGE, silver stain.; Purification: Chromatographic
• Form/Format: Sterile filtered, lyophilized.
• Sequence Length: 180

• Dimers/Aggregates: <1% by SDSPAGE
• Biological Activity: Fully biologically active compared to reference standards. Specific activity is 1 x 10^6 IU/mg.
• Endotoxin Level: <0.1 ng/ug
• Protein Content: Determined spectrophotometrically at 280nm and by RP-HPLC analysis against a calibrated standard.
• Solubility: Reconstitute the lyophilized product in sterile water not less than 100 ug/ml.
• Stabilizer: None
• Preservatives: None
• Preparation and Storage: Lyophilized product is stable at room temperature for 3 weeks. Store lyophilized product desiccated at -20 degree C. Upon reconstitution, store at 4 degree C for 2-7 days or aliquot and store at -20 degree C. For long-term storage add either HSA or BSA to 0.1%. Avoid repeated freeze-thaw cycles.

Related Product Information for IGF2 active protein

Recombinant Human IGF-II produced in E. coli is a single, non-glycosylated polypeptide chain containing 67 amino acids with a molecular mass of 7,505 daltons. This product has full biological activity.

Product Categories/Family for IGF2 active protein

Recombinant Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 189083846
• NCBI GeneID: 3481
• NCBI Accession #: NP_001121070.1
• NCBI GenBank Nucleotide #: NM_001127598.1
• UniProt Accession #: P01344; P78449; Q14299; Q1WM26; Q9UC68; Q9UC69; B3KX48; B7WP08
• Molecular Weight: 20,140 Da
• NCBI Official Full Name: insulin-like growth factor II isoform 2
• NCBI Official Synonym Full Names: insulin-like growth factor 2 (somatomedin A)
• NCBI Official Symbol: IGF2
• NCBI Official Synonym Symbols: IGF-II; PP9974; C11orf43; FLJ22066; FLJ44734
• NCBI Protein Information: insulin-like growth factor II; somatomedin-A; OTTHUMP00000011012; OTTHUMP00000011013; OTTHUMP00000011015; OTTHUMP00000011018; OTTHUMP00000011157; OTTHUMP00000011158; OTTHUMP00000220677; insulin-like growth factor type 2
• UniProt Protein Name: Insulin-like growth factor II
• Protein Family: Insulin-like growth factor
• UniProt Gene Name: IGF2
• UniProt Entry Name: IGF2_HUMAN

NCBI Description

This gene encodes a member of the insulin family of polypeptide growth factors, which are involved in development and growth. It is an imprinted gene, expressed only from the paternal allele, and epigenetic changes at this locus are associated with Wilms tumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and Silver-Russell syndrome. A read-through INS-IGF2 gene exists, whose 5' region overlaps the INS gene and the 3' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

IGF2: The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development. Epigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SRS). A clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations. The phenotypic expression changes during childhood and adolescence, with the facial features and asymmetry usually becoming more subtle with age. Belongs to the insulin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: extracellular space; extracellular region; plasma membrane

Molecular Function: protein serine/threonine kinase activator activity; insulin-like growth factor receptor binding; protein binding; growth factor activity; hormone activity; insulin receptor binding; receptor activator activity

Biological Process: positive regulation of catalytic activity; response to nicotine; genetic imprinting; multicellular organismal development; positive regulation of glycogen biosynthetic process; exocrine pancreas development; female pregnancy; positive regulation of activated T cell proliferation; response to estradiol stimulus; response to radiation; platelet degranulation; positive regulation of MAPKKK cascade; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; skeletal development; response to drug; response to nutrient levels; platelet activation; positive regulation of mitosis; glucose metabolic process; memory; osteoblast differentiation; positive regulation of protein kinase B signaling cascade; organ morphogenesis; positive regulation of peptidyl-tyrosine phosphorylation; response to ethanol; cellular protein metabolic process; insulin receptor signaling pathway; striated muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; positive regulation of insulin receptor signaling pathway; blood coagulation

Disease: Silver-russell Syndrome; Growth Restriction, Severe, With Distinctive Facies; Beckwith-wiedemann Syndrome; Wilms Tumor 1