Testing Data #1
(Both cells are apoptotic (green) and dying (blue nuclei). As the left cell is much brighter green than the right cell, the left cell had more active caspases)
CASPASE-8 Assay Kit | CASP8 assay kit
FAM FLICA CASPASE-8 KIT
Gene Names
CASP8; caspase-8
Applications
Flow Cytometry, Functional Assay, Immunofluorescence
Synonyms
CASPASE-8; FAM FLICA CASPASE-8 KIT; CASP8 assay kit
Form/Format
Kit
Sequence Length
482
Applicable Applications for CASP8 assay kit
Flow cytometry (FC/FACS), Immunofluorescence (IF)
Reagents In The Kits
4 vials of FAM-LETD-FMK FLICA Reagent - lyophilized
10X Wash Buffer, 60 mL
Fixative, 6 mL
Propidium Iodide, 1 mL
Hoechst Stain, 1 mL
10X Wash Buffer, 60 mL
Fixative, 6 mL
Propidium Iodide, 1 mL
Hoechst Stain, 1 mL
Preparation and Storage
Store the unopened kit (and each unopened component) at 4 degree C until the expiration date.
Protect the FLICA reagent from light at all times.
Once reconstituted, the 150X FLICA stock should be stored at -20 degree C protected from light.
Shelf Life: The 150X FLICA reconstituted stock is stable for up to 6 months and may be thawed twice during that time.
Protect the FLICA reagent from light at all times.
Once reconstituted, the 150X FLICA stock should be stored at -20 degree C protected from light.
Shelf Life: The 150X FLICA reconstituted stock is stable for up to 6 months and may be thawed twice during that time.
Testing Data #1
(Both cells are apoptotic (green) and dying (blue nuclei). As the left cell is much brighter green than the right cell, the left cell had more active caspases)
Testing Data #1
(Both cells are apoptotic (green) and dying (blue nuclei). As the left cell is much brighter green than the right cell, the left cell had more active caspases)
Related Product Information for CASP8 assay kit
Description: The FAM FLICA Caspase 8 kit uses a quick and easy method to analyze active caspases in apoptotic cells.
Test Principle: Caspase FLICA kits measure apoptosis by detecting active caspases in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a unique cell-permeable and non-cytotoxic reagent called the Fluorochrome Inhibitor of Caspases (FLICA). The FLICA reagent contains a caspase inhibitor sequence linked to a green (Carboxyfluorescein, FAM) fluorescent probe.
The Caspase FLICA Kits are suitable for cells in suspension, adherent cells, thin tissue sections (but not fixed or paraffin-embedded cells) from many species including mammalian, insect and yeast. Different cell types, e.g. HeLa, primary neurons, macrophages and lymphocytes have also been successfully studied with these kits. This kit can be used with a flow cytometer, fluorescence microscope or a fluorescence plate reader using black microtitre plates.
Test Principle: Caspase FLICA kits measure apoptosis by detecting active caspases in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a unique cell-permeable and non-cytotoxic reagent called the Fluorochrome Inhibitor of Caspases (FLICA). The FLICA reagent contains a caspase inhibitor sequence linked to a green (Carboxyfluorescein, FAM) fluorescent probe.
The Caspase FLICA Kits are suitable for cells in suspension, adherent cells, thin tissue sections (but not fixed or paraffin-embedded cells) from many species including mammalian, insect and yeast. Different cell types, e.g. HeLa, primary neurons, macrophages and lymphocytes have also been successfully studied with these kits. This kit can be used with a flow cytometer, fluorescence microscope or a fluorescence plate reader using black microtitre plates.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
caspase-8
NCBI Official Synonym Full Names
caspase 8, apoptosis-related cysteine peptidase
NCBI Official Symbol
CASP8
NCBI Official Synonym Symbols
caspase-8
NCBI Protein Information
caspase-8; caspase 8, apoptosis-related cysteine protease
Protein Family
Research Articles on CASP8
Similar Products
Product Notes
The CASP8 (Catalog #AAA239170) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CASPASE-8 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS), Immunofluorescence (IF). Researchers should empirically determine the suitability of the CASP8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CASPASE-8, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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