Typical Testing Data/Standard Curve (for reference only)
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Human Raftlin (RFTN1) ELISA Kit | RFTN1 elisa kit
Human Raftlin (RFTN1) ELISA Kit
Gene Names
RFTN1; MIG2; PIG9; PIB10; RAFTLIN
Reactivity
Human
Synonyms
Raftlin (RFTN1); Human Raftlin (RFTN1) ELISA Kit; RFTN1 elisa kit
Reactivity
Human
Sequence Length
578
Samples
Serum, plasma, cell culture supernates, ascites, tissue homogenates or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.5-40ng/ml
Sensitivity
0.41ng/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Related Product Information for RFTN1 elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Human Raftlin (also known as RFTN1) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human RFTN1 antibody. RFTN1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human RFTN1 Antibody is added and binds to RFTN1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated RFTN1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human RFTN1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human RFTN1 antibody. RFTN1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human RFTN1 Antibody is added and binds to RFTN1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated RFTN1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human RFTN1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
63,146 Da
NCBI Official Full Name
raftlin
NCBI Official Synonym Full Names
raftlin, lipid raft linker 1
NCBI Official Symbol
RFTN1
NCBI Official Synonym Symbols
MIG2; PIG9; PIB10; RAFTLIN
NCBI Protein Information
raftlin
UniProt Protein Name
Raftlin
Protein Family
UniProt Gene Name
RFTN1
UniProt Synonym Gene Names
KIAA0084
Uniprot Description
Involved in protein trafficking via association with clathrin and AP2 complex (PubMed:27022195, PubMed:21266579). Upon bacterial lipopolysaccharide stimulation, mediates internalization of TLR4 to endosomes in dendritic cells and macrophages; and internalization of poly(I:C) to TLR3-positive endosomes in myeloid dendritic cells and epithelial cells; resulting in activation of TICAM1-mediated signaling and subsequent IFNB1 production (PubMed:27022195, PubMed:21266579). Involved in T-cell antigen receptor-mediated signaling by regulating tyrosine kinase LCK localization, T-cell dependent antibody production and cytokine secretion (). May regulate B-cell antigen receptor-mediated signaling (PubMed:12805216). May play a pivotal role in the formation and/or maintenance of lipid rafts (PubMed:12805216).
Research Articles on RFTN1
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Product Notes
The Human RFTN1 rftn1 (Catalog #AAA1600006) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1600006 ELISA Kit recognizes Human RFTN1. It is sometimes possible for the material contained within the vial of "Raftlin (RFTN1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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