SDS-Page
(Fig.SDS-PAGE analysis of Mouse IL-4 protein.)
IL-4 active protein
Mouse IL-4 Protein
Gene Names
Il4; Il-4; BSF-1
Purity
>90% as determined by SDS-PAGE
Synonyms
IL-4; Mouse IL-4 Protein; Recombinant Mouse IL4 Protein (Interleukin-4) expressed in E. coli; BSF-1; Il-4; Interleukin-4; IL4; IL-4 active protein
Host
E Coli
Purity/Purification
>90% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH 7.4.
Sequence
Amino acid sequence derived from mouse IL4 (NP_067258.1) (His23-Ser140) was expressed with a N-terminal Met.
Sequence Length
140
Species
Mouse
Protein Length
The recombinant mouse IL4 consists of 119 amino acids and predicts a molecular mass of 13.4 KDa. It migrates as an approximately 14 KDa band in SDS-PAGE under reducing conditions.
Activity
Measured in a cell proliferation assay using HT-2 mouse T cells. The ED50 for this effect is typically 0.4-2 ng/mL. The ED50 for this effect is typically 0.4-2 ng/mL.
Usage Notes
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Mouse IL4 protein in sterile ddH2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Preparation and Storage
Item should be stored desiccated below -20 degree C. Upon reconstitution, the protein should be stored at 4 degree C between 2-7 days and for future use below -20 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
SDS-Page
(Fig.SDS-PAGE analysis of Mouse IL-4 protein.)
SDS-Page
(Fig.SDS-PAGE analysis of Mouse IL-4 protein.)
Related Product Information for IL-4 active protein
Interleukin-4, also known as IL4, is a secreted protein which belongs to the IL-4/ IL-13 family. Interleukin-4/ IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. It enhances both secretion and cell surface expression of IgE and IgG1. Interleukin-4/ IL4 also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Interleukin-4 is essential for the switching of B cells to IgE antibody production and for the maturation of T helper (Th) cells toward the Th2 phenotype. It participates in at least several B-cell activation processes as well as of other cell types. However, studies show that double mutant (Q116D, Y119D) of the murine IL4 protein (QY), both glutamine 116 and tyrosine 119, which binds to the IL4 receptor alpha, completely inhibites in a dose-dependent manner the IL4-induced proliferation of lipopolysaccharide-stimulated murine splenic B-cells, of the murine T cell line CTLL-2, and of the murine pre-B-cell line BA/F3. QY also inhibited the IL4-stimulated up-regulation of CD23 expression by lipopolysaccharide-stimulated murine splenic B-cells and abolished tyrosine phosphorylation of the transcription factor Stat6 and the tyrosine kinase Jak3 in IL4-stimulated BA/F3 cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
13.4 kDa
NCBI Official Full Name
interleukin-4
NCBI Official Synonym Full Names
interleukin 4
NCBI Official Symbol
Il4
NCBI Official Synonym Symbols
Il-4; BSF-1
NCBI Protein Information
interleukin-4
Research Articles on IL-4
Similar Products
Product Notes
The IL-4 (Catalog #AAA9718728) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Amino acid sequence derived from mouse IL4 (NP_067258.1) (His23-Ser 140) was expressed with a N-terminal Met. It is sometimes possible for the material contained within the vial of "IL-4, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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