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SDS-Page (Fig.SDS-PAGE analysis of Mouse IL-17 protein.)

IL-17 active protein

Mouse IL-17 Protein

Gene Names
Il17a; Il17; Ctla8; IL-17; Ctla-8; IL-17A
Purity
>97% as determined by SDS-PAGE
Synonyms
IL-17; Mouse IL-17 Protein; Recombinant Mouse IL17 Protein (Interleukin-17A) expressed in E. coli; Ctla-8; Ctla8; IL-17A; Il17; Interleukin-17A; IL17A; IL-17 active protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
>97% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH 7.4
Sequence
Amino acid sequence derived from mature form of mouse IL17a (Q62386) (Thr 22-Ala158) was expressed and purified.
Sequence Length
158
Species
Mouse
Protein Length
The recombinant mouse IL17a consists of 138 amino acids and predicts a molecular mass of 15.5 KDa. It migrates as an approximately 16 KDa band in SDS-PAGE under reducing conditions.
Activity
Measured by its binding ability in a functional ELISA. Measured by its ability to induce IL-6 secretion by NIH?3T3 mouse embryonic fibroblast cells in the presense of 20 ng/mL TNFalpha. The ED50 for this effect is 0.15-0.6 ng/mL. 2. Immobilized recombinant mouse IL17a (Cat:51065-MNAE) at 10 mug/mL (100 mul/well) can bind human IL17Ra-Fc2h (Cat:10895-H03H) with a linear range of 16-500 ng/mL.
Usage Notes
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Mouse IL17 protein in sterile ddH2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Preparation and Storage
Item should be stored desiccated below -20 degree C. Upon reconstitution, the protein should be stored at 4 degree C between 2-7 days and for future use below -20 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

SDS-Page

(Fig.SDS-PAGE analysis of Mouse IL-17 protein.)

SDS-Page (Fig.SDS-PAGE analysis of Mouse IL-17 protein.)
Related Product Information for IL-17 active protein
IL17, also known as IL17a, is a cytokine belongs to the IL-17 family. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. The IL-17 family of cytokines includes six members, IL-17/IL-17A, IL-17B, IL-17C, IL-17D, IL-17E/IL-25, and IL-17F, which are produced by multiple cell types. IL-17 regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
15.5 kDa
NCBI Official Full Name
interleukin-17A
NCBI Official Synonym Full Names
interleukin 17A
NCBI Official Symbol
Il17a
NCBI Official Synonym Symbols
Il17; Ctla8; IL-17; Ctla-8; IL-17A
NCBI Protein Information
interleukin-17A
UniProt Protein Name
Interleukin-17A
UniProt Gene Name
Il17a
UniProt Synonym Gene Names
Ctla8; Il17; IL-17; IL-17A; CTLA-8
UniProt Entry Name
IL17_MOUSE

NCBI Description

This gene encodes a pro-inflammatory cytokine that is a member of the interleukin-17 family. The encoded protein plays a central role in host defense against diverse pathogens. The encoded protein is produced by activated T-cells and certain cell types of innate immune system. The active protein functions as either a homodimer with other interleukin-17 family members and signals through the interleukin-17 receptor to induce inflammatory cytokine production. Aberrant expression of this gene is associated with autoinflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. [provided by RefSeq, Sep 2015]

Uniprot Description

IL17A: Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts. Belongs to the IL-17 family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular space; cytoplasm; extracellular region; external side of plasma membrane

Molecular Function: cytokine activity

Biological Process: positive regulation of osteoclast differentiation; positive regulation of interleukin-23 production; positive regulation of transcription from RNA polymerase II promoter; inflammatory response

Research Articles on IL-17

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Product Notes

The IL-17 il17a (Catalog #AAA9718727) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Amino acid sequence derived from mature form of mouse IL17a (Q62386) (Thr 22-Ala158) was expressed and purified. It is sometimes possible for the material contained within the vial of "IL-17, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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