IL-17 active protein

Human IL-17 Protein

Cat. Num. AAA9718710

• Gene Names: IL17A; IL17; CTLA8; IL-17; CTLA-8; IL-17A
• Purity: >97% as determined by SDS-PAGE
• Synonyms: IL-17; Human IL-17 Protein; Recombinant Human IL17 Protein (Interleukin-17A) expressed in E. coli; CTLA-8; CTLA8; IL-17A; IL17; Interleukin-17A; IL17A; IL-17 active protein

• Host: E Coli
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence: Amino acid sequence derived from mature form of human IL17A (Q16552) (Ile20-Ala155) was expressed, with a N-terminal Met.
• Sequence Length: 155

• Species: Human
• Protein Length: The recombinant human IL17A consists of 137 amino acids and predicts a molecular mass of 15.7 KDa. It migrates as an approximately 16 KDa band in SDS-PAGE under reducing conditions.
• Activity: Measured by its ability to induce IL-6 secretion by NIH?3T3 mouse embryonic fibroblast cells in the presense of 20 ng/mL TNFalpha. The ED50for this effect is 0.5-2.5 ng/mL.
• Usage Notes: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL17 protein in sterile ddH2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Item should be stored desiccated below -20 degree C. Upon reconstitution, the protein should be stored at 4 degree C between 2-7 days and for future use below -20 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

SDS-Page

(Fig.SDS-PAGE analysis of Human IL-17 protein.)

Related Product Information for IL-17 active protein

IL17, also known as IL17a, is a cytokine belongs to the IL-17 family. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. The IL-17 family of cytokines includes six members, IL-17/IL-17A, IL-17B, IL-17C, IL-17D, IL-17E/IL-25, and IL-17F, which are produced by multiple cell types. IL-17 regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.

NCBI and Uniprot Product Information

• NCBI GI #: 4504651
• NCBI GeneID: 3605
• NCBI Accession #: NP_002181.1
• NCBI GenBank Nucleotide #: NP_002181.1
• UniProt Accession #: Q16552
• Molecular Weight: 15.7 kDa
• NCBI Official Full Name: interleukin-17A
• NCBI Official Synonym Full Names: interleukin 17A
• NCBI Official Symbol: IL17A
• NCBI Official Synonym Symbols: IL17; CTLA8; IL-17; CTLA-8; IL-17A
• NCBI Protein Information: interleukin-17A
• UniProt Protein Name: Interleukin-17A
• UniProt Gene Name: IL17A
• UniProt Synonym Gene Names: CTLA8; IL17; IL-17; IL-17A; CTLA-8
• UniProt Entry Name: IL17_HUMAN

NCBI Description

The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. [provided by RefSeq, Jul 2008]

Uniprot Description

IL17A: Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts. Belongs to the IL-17 family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 6p12

Cellular Component: extracellular space; external side of plasma membrane

Molecular Function: cytokine activity

Biological Process: cell death; cell-cell signaling; positive regulation of osteoclast differentiation; apoptosis; positive regulation of interleukin-23 production; protein amino acid glycosylation; positive regulation of transcription from RNA polymerase II promoter; immune response; inflammatory response