Rabbit anti-Human SEMA6A Polyclonal Antibody | anti-SEMA6A antibody

SEMA6A, NT (SEMA6A, KIAA1368, SEMAQ, Semaphorin-6A, Semaphorin VIA, Semaphorin-6A-1) (FITC)

Cat. Num. AAA6346023

• Gene Names: SEMA6A; VIA; SEMA; HT018; SEMAQ; SEMA6A1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SEMA6A; Polyclonal Antibody; NT (SEMA6A; KIAA1368; SEMAQ; Semaphorin-6A; Semaphorin VIA; Semaphorin-6A-1) (FITC); Semaphorin-6A-1; anti-SEMA6A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-SEMA6A antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SEMA6A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-65 amino acids from the N-terminal region of human SEMA6A.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SEMA6A Antibody (N-term) western blot analysis in A2058 cell line lysates (15ug/lane).This demonstrates the SEMA6A antibody detected the SEMA6A protein (arrow).)

Related Product Information for anti-SEMA6A antibody

SEMA6A belongs to a subfamily characterized by an extracellular semaphorin domain, a transmembrane domain, and a long cytoplasmic tail. Members of this class can repel sympathetic and dorsal root ganglion axons in vitro, consistent with a traditional role as guidance signals. However, the length of the cytoplasmic tail, which includes an EVL-binding site in SEMA6A and an Src-binding site in SEMA6B, suggests that these semaphorins may also function as receptors. SEMA6A is expressed in developing neural tissue and is required for proper development of the thalamocortical projection. SEMA6A directly links the Ena/VASP and the semaphorin protein families since the SEMA6A protein is capable of selective binding to the protein EVL (Ena/VASP-like protein).

Product Categories/Family for anti-SEMA6A antibody

Antibodies; Plexin; Semaphorin

References

Johnson, M.P., et al. Hum. Genet. 126(5):655-666(2009). Landers, J.E., et al. Proc. Natl. Acad. Sci. U.S.A. 106(22):9004-9009(2009). Prislei, S., et al. Mol. Cancer Ther. 7(1):233-241(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 11991660
• NCBI GeneID: 57556
• NCBI Accession #: NP_065847.1
• NCBI GenBank Nucleotide #: NM_020796.4
• UniProt Accession #: Q9H2E6; Q9P2H9
• Molecular Weight: 116,336 Da
• NCBI Official Full Name: semaphorin-6A isoform 2
• NCBI Official Synonym Full Names: semaphorin 6A
• NCBI Official Symbol: SEMA6A
• NCBI Official Synonym Symbols: VIA; SEMA; HT018; SEMAQ; SEMA6A1
• NCBI Protein Information: semaphorin-6A
• UniProt Protein Name: Semaphorin-6A
• Protein Family: Semaphorin
• UniProt Gene Name: SEMA6A
• UniProt Synonym Gene Names: KIAA1368; SEMAQ; Sema VIA; SEMA6A-1

NCBI Description

The transmembrane semaphorin SEMA6A is expressed in developing neural tissue and is required for proper development of the thalamocortical projection (Leighton et al., 2001 [PubMed 11242070]).[supplied by OMIM, Feb 2011]

Uniprot Description

Cell surface receptor for PLXNA2 that plays an important role in cell-cell signaling. Required for normal granule cell migration in the developing cerebellum. Promotes reorganization of the actin cytoskeleton and plays an important role in axon guidance in the developing central nervous system. Can act as repulsive axon guidance cue. Has repulsive action towards migrating granular neurons. May play a role in channeling sympathetic axons into the sympathetic chains and controlling the temporal sequence of sympathetic target innervation ().