GOT2 active protein

GOT2, human recombinant

Cat. Num. AAA844883

• Gene Names: Got2; Got-2; mAspAT; FABP-pm; AL022787
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: GOT2; human recombinant; Aspartate aminotransferase 2; mitochondrial; KAT4; KATIV; mitAAT; GOT2 active protein

• Host: E Coli
• Purity/Purification: >=95%
• Form/Format: 0.5 mg/ml solution 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 150 mM NaCl and 1 mM DTT.; Appearance: Liquid
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSSSWWTHV EMGPPDPILG VTEAFKRDTN SKKMNLGVGA YRDDNGKPYV LPSVRKAEAQ IAAKNLDKEY LPIGGLAEFC KASAELALGE NSEVLKSGRF VTVQTISGTG ALRIGASFLQ RFFKFSRDVF LPKPTWGNHT PIFRDAGMQL QGYRYYDPKT CGFDFTGAVE DISKIPEQSV LLLHACAHNP TGVDPRPEQW KEIATVVKKR NLFAFFDMAY QGFASGDGDK DAWAVRHFIE QGINVCLCQS YAKNMGLYGE RVGAFTMVCK DADEAKRVES QLKILIRPMY SNPPLNGARI AAAILNTPDL RKQWLQEVKV MADRIIGMRT QLVSNLKKEG STHNWQHITD QIGMFCFTGL KPEQVERLIK EFSIYMTKDG RISVAGVTSS NVGYLAHAIH QVTK
• Sequence Length: 430

• Applicable Applications for GOT2 active protein: SDS-PAGE
• Biological Activity: Specific activity is > 44 unit/mg, in which one unit will convert 1.0 umole of alpha-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25 degree C.
• Results: Specific activity is > 44 unit/mg
• Unit Definition: One unit will convert 1.0 umole of alpha-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25 degree C.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for GOT2 active protein

Background: GOT2, also known as glutamate oxaloacetate transaminase 2, belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Glutamate oxaloacetate transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Recombinant human GOT2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Product Categories/Family for GOT2 active protein

Proteins and Enzymes; Proteins and Enzymes (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 6754036
• NCBI GeneID: 14719
• NCBI Accession #: NP_034455.1
• NCBI GenBank Nucleotide #: NM_010325.2
• UniProt Accession #: P05202; O09188; Q3TIP6; Q3UD91; Q5HZH5
• Molecular Weight: 48.4 kDa (433 aa, 1-413 aa + His Tag)
• NCBI Official Full Name: aspartate aminotransferase, mitochondrial
• NCBI Official Synonym Full Names: glutamatic-oxaloacetic transaminase 2, mitochondrial
• NCBI Official Symbol: Got2
• NCBI Official Synonym Symbols: Got-2; mAspAT; FABP-pm; AL022787
• NCBI Protein Information: aspartate aminotransferase, mitochondrial
• UniProt Protein Name: Aspartate aminotransferase, mitochondrial
• UniProt Gene Name: Got2
• UniProt Synonym Gene Names: Got-2; mAspAT; FABP-1; FABPpm
• UniProt Entry Name: AATM_MOUSE

Uniprot Description

GOT2: Catalyzes the irreversible transamination of the L- tryptophan metabolite L-kynurenine to form kynurenic acid (KA). Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; EC 2.6.1.1; Amino Acid Metabolism - cysteine and methionine; Transferase; Amino Acid Metabolism - phenylalanine; Mitochondrial; Amino Acid Metabolism - tyrosine; EC 2.6.1.7

Cellular Component: protein complex; cell surface; mitochondrion; membrane; mitochondrial inner membrane; plasma membrane; perikaryon; myelin sheath

Molecular Function: transferase activity; amino acid binding; protein homodimerization activity; enzyme binding; kynurenine-oxoglutarate transaminase activity; carboxylic acid binding; aspartate transaminase activity; phospholipid binding; transaminase activity; catalytic activity; pyridoxal phosphate binding

Biological Process: amino acid metabolic process; dicarboxylic acid metabolic process; response to ethanol; oxaloacetate metabolic process; transport; glutamate catabolic process to aspartate; aspartate biosynthetic process; aspartate catabolic process; aspartate metabolic process; biosynthetic process; glutamate catabolic process to 2-oxoglutarate; lipid transport