Rabbit anti-Human NANOS1 Polyclonal Antibody | anti-NANOS1 antibody

NANOS1, ID (NANOS1, NOS1, Nanos homolog 1, EC_Rep1a)

Cat. Num. AAA648789

• Gene Names: NANOS1; NOS1; SPGF12
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: NANOS1; Polyclonal Antibody; ID (NANOS1; NOS1; Nanos homolog 1; EC_Rep1a); Anti -NANOS1; anti-NANOS1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-NANOS1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:10-50
• Immunogen: NANOS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-124 amino acids from the Central region of human NANOS1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(NANOS1 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the NANOS1 antibody detected the NANOS1 protein (arrow).)

Immunohistochemistry (IHC)

(NANOS1 Antibody (Center) (AP14401c)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NANOS1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-NANOS1 antibody

NANOS1 may regulate translation of specific mRNAs by forming a complex with PUM2 that associates with the 3'-UTR of mRNA targets.

Product Categories/Family for anti-NANOS1 antibody

Antibodies; Abs to DNA/RNA Translation

NCBI and Uniprot Product Information

• NCBI GI #: 40804761
• NCBI GeneID: 340719
• NCBI Accession #: NP_955631.1
• NCBI GenBank Nucleotide #: NM_199461.2
• UniProt Accession #: Q8WY41
• Molecular Weight: 30,230 Da
• NCBI Official Full Name: nanos homolog 1
• NCBI Official Synonym Full Names: nanos homolog 1 (Drosophila)
• NCBI Official Symbol: NANOS1
• NCBI Official Synonym Symbols: NOS1; SPGF12
• NCBI Protein Information: nanos homolog 1; NOS-1; EC_Rep1a
• UniProt Protein Name: Nanos homolog 1
• Protein Family: Nanos
• UniProt Gene Name: NANOS1
• UniProt Synonym Gene Names: NOS1; NOS-1
• UniProt Entry Name: NANO1_HUMAN

NCBI Description

This gene encodes a CCHC-type zinc finger protein that is a member of the nanos family. This protein co-localizes with the RNA-binding protein pumilio RNA-binding family member 2 and may be involved in regulating translation as a post-transcriptional repressor. Mutations in this gene are associated with spermatogenic impairment. [provided by RefSeq, Sep 2015]

Uniprot Description

Function: May act as a translational repressor which regulates translation of specific mRNAs by forming a complex with PUM2 that associates with the 3'-UTR of mRNA targets. Capable of interfering with the proadhesive and anti-invasive functions of E-cadherin. Up-regulates the production of MMP14 to promote tumor cell invasion. Ref.2 Ref.4

Subunit structure: Interacts with PUM2, SNAPIN and CTNNB1. Interacts (via N-terminal region) with CTNND1. Ref.1 Ref.2 Ref.5

Subcellular location: Cytoplasm › perinuclear region. Cytoplasm. Note: Colocalizes with SNAPIN and PUM2 in the perinuclear region of germ cells. Ref.1 Ref.2 Ref.5

Tissue specificity: Testis and ovary (at protein level). Predominantly expressed in testis. Specifically expressed during germline development. In adult tissues, it is mainly expressed in spermatogonia, the stem cells of the germline. Also expressed during meiosis in spermatocytes. Not present in late, post-meiotic stage germ cells. Expressed in fetal ovaries, while it is weakly or not expressed in mature postmeiotic oocytes, suggesting that it may be expressed in premeiotic female germ cells. Expressed at high levels only in the E-cadherin deficient cell lines. Highly expressed in lung carcinomas and mostly detected in invasive tumor cells and its expression correlates with tumor aggressiveness. Ref.1 Ref.2 Ref.4 Ref.5 Ref.6

Developmental stage: Fetal ovary and fetal testis (at protein level). Ref.6

Induction: Down-regulated by E-cadherin. Ref.2

Domain: The Nanos-type zinc finger is composed of two C2HC motifs, each motif binding one molecule of zinc. It is essential for the translation repression activity of the protein

By similarity.The N-terminal region and C-terminal zinc-finger RNA-binding domain are both necessary for interaction with SNAPIN.

Sequence similarities: Belongs to the nanos family.Contains 1 nanos-type zinc finger.