Rabbit anti-Mouse, Rat NFkappaB p50 Polyclonal Antibody | anti-NFKB1 antibody

NFkappaB p50 polyclonal antibody

Cat. Num. AAA565251

• Gene Names: Nfkb1; p50; p105; NF-KB1; p50/p105; NF-kappaB; NF-kappaB1
• Reactivity: Mouse, Rat
• Applications: Western Blot
• Purity: Peptide Affinity Purified
• Synonyms: NFkappaB p50; Polyclonal Antibody; NFkappaB p50 polyclonal antibody; anti-NFKB1 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes mouse and rat NF-kappaB p50. Detects bands of ~50kDa and ~105kDa by Western blot.
• Purity/Purification: Peptide Affinity Purified
• Form/Format: Liquid. In borate buffered saline, pH 8.2.
• Sequence Length: 971

• Applicable Applications for anti-NFKB1 antibody: Western Blot (WB)
• Application Notes: Western Blot (0.5ug/ml, colorimetric); Suggested dilutions/conditions may not be available for all applications.; Detects bands of ~50kDa and ~105kDa by Western blot.
• Immunogen: Synthetic peptide corresponding to the sequence near the N-terminus of mouse NF-kappaB.
• Preparation and Storage: Long term storage: -20 degree C; Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of rat spleen tissue extract, probed with NF-kappaB (p50) pAb.)

Related Product Information for anti-NFKB1 antibody

Nuclear factor kappaB (NF-kappaB) was identified as a sequence specific transcriptional activator that binds to the intronic enhancer of the kappa light chain gene in B lymphocytes. NF-kappaB is a heterodimer that consists of a 50 kDa DNA binding subunit (p50) and a 65 kDa transactivation subunit (p65/RelA). The p50/p65 heterodimer remains in the cytosol in an inactive form as a complex with its inhibitor, IkappaB. Upon stimulation of cells by a wide variety of stimuli such as lipopolysaccharide (LPS), pro-inflammatory cytokines, and viral infection, IkappaB is phosphorylated and degraded by the proteosome. The active NF-kappaB heterodimer is translocated into the nucleus and induces gene expression.

Product Categories/Family for anti-NFKB1 antibody

DNA Regulation & Transcription; Cell Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 341941170
• NCBI GeneID: 18033
• UniProt Accession #: P25799; Q3TZE8; Q3V2V6; Q6TDG8; Q75ZL1; Q80Y21; Q8C712; B2RRQ6
• Molecular Weight: 43,983 Da
• NCBI Official Full Name: Nuclear factor NF-kappa-B p105 subunit
• NCBI Official Synonym Full Names: nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105
• NCBI Official Symbol: Nfkb1
• NCBI Official Synonym Symbols: p50; p105; NF-KB1; p50/p105; NF-kappaB; NF-kappaB1
• NCBI Protein Information: nuclear factor NF-kappa-B p105 subunit
• UniProt Protein Name: Nuclear factor NF-kappa-B p105 subunit
• Protein Family: Nuclear factor
• UniProt Gene Name: Nfkb1
• UniProt Entry Name: NFKB1_MOUSE

Uniprot Description

NFkB-p105: a transcription factor of the nuclear factor-kappaB ( NFkB) group. Undergoes cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of NFkB. NFkB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFkB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFkB has been associated with a number of inflammatory diseases while persistent inhibition of NFkB leads to inappropriate immune cell development or delayed cell growth. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Two alternatively spliced isoforms have been described.

Protein type: DNA-binding; Transcription factor

Cellular Component: cytoplasm; cytosol; mitochondrion; neuron projection; nucleoplasm; nucleus; protein complex

Molecular Function: actinin binding; chromatin binding; DNA binding; double-stranded DNA binding; heat shock protein binding; identical protein binding; protein binding; protein heterodimerization activity; protein homodimerization activity; sequence-specific DNA binding; transcription factor activity

Biological Process: apoptosis; I-kappaB kinase/NF-kappaB cascade; inflammatory response; innate immune response; lymph node development; negative regulation of apoptosis; negative regulation of cytokine production; negative regulation of inflammatory response; negative regulation of interleukin-12 biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; response to cytokine stimulus; response to organic cyclic substance; response to oxidative stress; signal transduction; transcription, DNA-dependent