Rabbit anti-Human BCMO1 Polyclonal Antibody | anti-BCMO1 antibody

BCMO1, ID (BCMO1, BCDO, BCDO1, Beta,beta-carotene 15,15'-monooxygenase, Beta-carotene dioxygenase 1) (AP)

Cat. Num. AAA6278065

• Gene Names: BCO1; BCO; BCDO; BCMO; BCDO1; BCMO1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: BCMO1; Polyclonal Antibody; ID (BCMO1; BCDO; BCDO1; Beta; beta-carotene 15; 15'-monooxygenase; Beta-carotene dioxygenase 1) (AP); Beta-carotene dioxygenase 1; anti-BCMO1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-BCMO1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: BCMO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 270-300 amino acids from the Central region of human BCMO1.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(BCMO1 Antibody (Center) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the BCMO1 antibody detected the BCMO1 protein (arrow).)

Related Product Information for anti-BCMO1 antibody

Vitamin A metabolism is important for vital processes such as vision, embryonic development, cell differentiation, and membrane and skin protection. The protein encoded by this gene is a key enzyme in beta-carotene metabolism to vitamin A. It catalyzes the oxidative cleavage of beta,beta-carotene into two retinal molecules.

Product Categories/Family for anti-BCMO1 antibody

Antibodies; Enzymes; Oxidase

References

Lietz, G., et al. Arch. Biochem. Biophys. 502(1):8-16(2010). Kim, Y.S., et al. Biotechnol. Lett. 32(6):847-853(2010). Dastani, Z., et al. Eur. J. Hum. Genet. 18(3):342-347(2010). Perry, J.R., et al. Diabetologia 52(10):2117-2121(2009). Ferrucci, L., et al. Am. J. Hum. Genet. 84(2):123-133(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 74027270
• NCBI GeneID: 53630
• NCBI Accession #: NP_059125.2
• NCBI GenBank Nucleotide #: NM_017429.2
• UniProt Accession #: Q9HAY6; Q9NVH5; A0AV48; A0AV50
• Molecular Weight: 62,637 Da
• NCBI Official Full Name: beta,beta-carotene 15,15'-monooxygenase
• NCBI Official Synonym Full Names: beta-carotene oxygenase 1
• NCBI Official Symbol: BCO1
• NCBI Official Synonym Symbols: BCO; BCDO; BCMO; BCDO1; BCMO1
• NCBI Protein Information: beta,beta-carotene 15,15'-monooxygenase; beta-carotene dioxygenase 1; beta-carotene 15, 15'-dioxygenase 1; beta-carotene 15,15'-monooxygenase 1
• UniProt Protein Name: Beta,beta-carotene 15,15'-monooxygenase
• UniProt Gene Name: BCMO1
• UniProt Synonym Gene Names: BCDO; BCDO1
• UniProt Entry Name: BCDO1_HUMAN

NCBI Description

Vitamin A metabolism is important for vital processes such as vision, embryonic development, cell differentiation, and membrane and skin protection. The protein encoded by this gene is a key enzyme in beta-carotene metabolism to vitamin A. It catalyzes the oxidative cleavage of beta,beta-carotene into two retinal molecules. [provided by RefSeq, Jul 2008]

Uniprot Description

BCMO1: Symmetrically cleaves beta-carotene into two molecules of retinal. The reaction proceeds in three stages, epoxidation of the 15,15'-double bond, hydration of the double bond leading to ring opening, and oxidative cleavage of the diol formed. Defects in BCMO1 are the cause of autosomal dominant hypercarotenemia and vitamin A deficiency (ADHVAD). Vitamin A is essential for normal embryonic development as well as normal physiological functions in children and adults. Hypercarotenemia is characterized by an excess carotene in the serum, but unlike excess vitamin A, carotene is non-toxic. So far, only a few cases of excess vitamin A have been reported. Individuals were thought to be vitamin A deficient due to an impairment in the conversion of carotenoids to retinal in the intestine. Belongs to the carotenoid oxygenase family.

Protein type: Cofactor and Vitamin Metabolism - retinol; EC 1.14.99.36; Oxidoreductase

Chromosomal Location of Human Ortholog: 16q23.2

Cellular Component: cytosol

Molecular Function: beta-carotene 15,15'-monooxygenase activity; metal ion binding; monooxygenase activity

Biological Process: phototransduction, visible light; vitamin A biosynthetic process; retinal metabolic process; retinol metabolic process; retinoid metabolic process

Disease: Hypercarotenemia And Vitamin A Deficiency, Autosomal Dominant