GDF11 active protein

GDF11 protein

Cat. Num. AAA538017

• Gene Names: GDF11; BMP11; BMP-11
• Applications: User optimized.
• Purity: > 98% by SDS-PAGE analysis and HPLC
• Synonyms: GDF11; GDF11 protein; BMP-11 protein; GDF 11 protein; GDF-11 protein; GDF-11; Growth/Differentiation Factor-11 protein; GDF 11; GDF11 active protein

• Host: E. coli
• Purity/Purification: > 98% by SDS-PAGE analysis and HPLC
• Form/Format: Sterile Filtered through a 0.2 micron filter. Lyophilized with no additives. Centrifuge vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0mg/ml. Keep pH below 5.0. Do not vortex.
• Concentration: 20 ug per vial: lyophilized (varies by lot)
• Sequence: Region of GDF11 protein corresponding to amino acids NLGLDCDEHS SESRCCRYPL TVDFEAFGWD WIIAPKRYKA NYCSGQCEYM FMQKYPHTHL VQQANPRGSA GPCCTPTKMS PINMLYFNDK QQIIYGKIPG MVVDRCGCS.

• Applicable Applications for GDF11 active protein: User optimized.
• Grade: Recombinant
• Species: Human
• Contaminants: Endotoxin level is <0.1ng/ug
• Biological Activity: The biological activity is determined by its ability to inhibit alkaline phosphatase activity in differentiating MC3T3/E1 cells. The ED₅₀ for this effect is 8 to 10 ng/ml.
• Biohazard: Use normal laboratory precautions and techniques when handling this reagent.
• Preparation and Storage: Ships ambient or refrigerated. Upon receipt store at -20°C. After reconstituted, the undiluted solution is stable at 4°C for up to 1 week. For extended storage, dilute further in a buffer containing a carrier protein (0.1% BSA) and store in working aliquots at -20°C to -80°C. Avoid repeat freeze-thaw cycles.

Related Product Information for GDF11 active protein

GDF-11 is a myostatin-homologous protein that acts as an inhibitor of nerve tissue growth. GDF-11 has been shown to suppress neurogenesis through a myostatin-like pathway, which involves arrest of progenitor cell-cycle in the G1 phase. Similarities between myostatin and GDF-11, which are 90% identical in their amino acid sequence, suggests that the regulatory mechanisms responsible for maintaining proper tissue size during neural and muscular development might be the same.

Product Categories/Family for GDF11 active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 5031613
• NCBI GeneID: 10220
• NCBI Accession #: NP_005802.1
• NCBI GenBank Nucleotide #: NM_005811.3
• UniProt Accession #: O95390; Q9UID1; Q9UID2
• Molecular Weight: 25kDa
• NCBI Official Full Name: growth/differentiation factor 11
• NCBI Official Synonym Full Names: growth differentiation factor 11
• NCBI Official Symbol: GDF11
• NCBI Official Synonym Symbols: BMP11; BMP-11
• NCBI Protein Information: growth/differentiation factor 11
• UniProt Protein Name: Growth/differentiation factor 11
• Protein Family: Growth/differentiation factor
• UniProt Gene Name: GDF11
• UniProt Synonym Gene Names: BMP11; GDF-11; BMP-11
• UniProt Entry Name: GDF11_HUMAN

NCBI Description

The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in mice and Xenopus suggest that this protein is involved in mesodermal formation and neurogenesis during embryonic development. [provided by RefSeq, Jul 2008]

Uniprot Description

GDF11: Secreted signal that acts globally to specify positional identity along the anterior/posterior axis during development. Play critical roles in patterning both mesodermal and neural tissues and in establishing the skeletal pattern. Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12q13.2

Cellular Component: extracellular space

Molecular Function: growth factor activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: nervous system development; cell maturation; palate development; regulation of apoptosis; negative regulation of cell proliferation; negative regulation of cell differentiation; camera-type eye morphogenesis; spinal cord anterior/posterior patterning; ureteric bud development; regulation of MAPKKK cascade; pancreas development; mesoderm development; metanephros development; skeletal development; cell development; growth