G-CSF active protein

Recombinant Human G-CSF

Cat. Num. AAA225725

• Gene Names: CSF3; GCSF; CSF3OS; C17orf33
• Applications: Functional Assay
• Purity: >=98% determined by silver staining of SDS-PAGE gel; Purified by ion exchange chromatography
• Synonyms: G-CSF; Recombinant Human G-CSF; G-CSF active protein

• Host: E Coli
• Purity/Purification: >=98% determined by silver staining of SDS-PAGE gel; Purified by ion exchange chromatography
• Form/Format: Lyophilized
• Concentration: 500 mug/ml after reconstitution (varies by lot)
• Sequence Length: 168

• Applicable Applications for G-CSF active protein: Functional Assay
• Source: Human
• Endotoxin Level: <1 EU/mug
• Activity: Confirmed by performing an alamarBlue based cell proliferation assay using mouse myelogenous leukemia lymphoblast cells. The expected ED50 for this effect is 10-70 pg/ml.
• Pre-Lyophilization Buffer: 20 mM Phosphate Buffer, 0.1 M Sodium Chloride
• Preservative Stabilizers: 1.0% Trehalose
• Reconstitution: Pack Size: 10 ug; Centrifuge vial prior to reconstitution. Reconstitute to 500 mug/ml by adding 20 mul ddH2O.; Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. Do not vortex.; Pack Size: 100 ug; Centrifuge vial prior to reconstitution. Reconstitute to 500 ug/ml by adding 200 mul ddH2O.; Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. Do not vortex.
• Preparation and Storage: Prior to reconstitution store at -20 degree C. Following reconstitution store at -20 degree C.; This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein.; Should this product contain a precipitate we recommend microcentrifugation before use.

Testing Data

(Silver Staining of SDS-PAGE gelPrecision Plus Protein Kaleidoscope Prestained Protein Standards were run in lane 1 and 1 mug Recombinant Human G-CSF was run in lane 2 on a 4-15% Mini-PROTEAN TGX Stain-Free Protein Gel. Silver staining was performed using the Silver Stain Plus Kit)

Testing Data

(The proliferative effect of recombinant human G-CSF was demonstrated by performing a cell proliferation assay with mouse myelogenous leukemia lymphoblast cells using alamarBlue (BUF012A). The expected ED50 for this effect is 10-70 pg/ml)

Related Product Information for G-CSF active protein

Recombinant Human G-CSF Granulocyte colony stimulating factor (G-CSF) is a member of the colony-stimulating factor hematopoietic cytokine family (Cavalcante et al. 2015). Similar to other cytokines, G-CSF plays a critical role in the immune response to infection. G-CSF is expressed by a number of cell types including monocytes, macrophages and fibroblasts (Panopoulos and Watowich 2008).
Protein levels are low in healthy individuals however increase significantly upon inflammatory stimuli such as interleukin 1 and TNF-alpha (Christensen et al. 2016, Panopoulos and Watowich 2008). G-CSF regulates neutrophilic granulocytes by stimulating neutrophil proliferation, differentiation, survival and also plays a key role in neutrophil mobilization into the bloodstream (Cavalcante et al. 2015).
G-CSF signaling is mediated by binding to the G-CSF receptor (G-CSFR, CD114 in humans), which is expressed by a number of cell types including myeloid leukemic cells, mature neutrophils, platelets, monocytes and cardiomyocytes (Panopoulos and Watowich 2008). Upon ligand binding, G-CSFR dimerizes, which results in receptor phosphorylation and subsequent activation of a number of cell signaling pathways, including JAK/STAT and Ras-MAPK signaling pathways (Tamada et al. 2005, Avalos 1996).
As a result of chemotherapy, cancer patients commonly develop neutropenia (Crawford et al. 2004). G-CSF is the active ingredient of drugs used to treat chemotherapy associated neutropenia (Lustberg 2012). G-CSF exacerbates inflammatory conditions such as rheumatoid arthritis; Eyles et al. (2008) suggest modulation of G-CSF as a potential therapy route.
The proliferative effect of G-CSF was demonstrated by performing a cell proliferation assay with NFS?60 mouse myelogenous leukemia lymphoblast cells. The expected ED50 for this effect is 10-70 pg/ml.

NCBI and Uniprot Product Information

• NCBI GI #: 296011057
• NCBI GeneID: 1440
• NCBI Accession #: NP_001171618.1
• NCBI GenBank Nucleotide #: NM_001178147.1
• UniProt Accession #: P09919
• Molecular Weight: 18.7 kDa
• NCBI Official Full Name: granulocyte colony-stimulating factor isoform d
• NCBI Official Synonym Full Names: colony stimulating factor 3
• NCBI Official Symbol: CSF3
• NCBI Official Synonym Symbols: GCSF; CSF3OS; C17orf33
• NCBI Protein Information: granulocyte colony-stimulating factor
• UniProt Protein Name: Granulocyte colony-stimulating factor
• UniProt Gene Name: CSF3
• UniProt Synonym Gene Names: C17orf33; GCSF; G-CSF
• UniProt Entry Name: CSF3_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]

Uniprot Description

G-CSF: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. Belongs to the IL-6 superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Cell cycle regulation; Cytokine; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q11.2-q12

Cellular Component: extracellular space

Molecular Function: enzyme binding; growth factor activity; cytokine activity; granulocyte colony-stimulating factor receptor binding

Biological Process: granulocyte differentiation; positive regulation of myeloid cell differentiation; positive regulation of protein binding; multicellular organismal development; cytokine and chemokine mediated signaling pathway; positive regulation of peptidyl-serine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of actin filament polymerization; positive regulation of cell proliferation; positive regulation of transcription factor import into nucleus; immune response; positive regulation of transcription from RNA polymerase II promoter