Rabbit anti-Human FKBP14 Polyclonal Antibody | anti-FKBP14 antibody

FKBP14, CT (FKBP14, FKBP22, Peptidyl-prolyl cis-trans isomerase FKBP14, 22kD FK506-binding protein, FK506-binding protein 14, Rotamase) (Azide free) (HRP)

Cat. Num. AAA6299345

• Gene Names: FKBP14; EDSKMH; FKBP22; IPBP12
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FKBP14; Polyclonal Antibody; CT (FKBP14; FKBP22; Peptidyl-prolyl cis-trans isomerase FKBP14; 22kD FK506-binding protein; FK506-binding protein 14; Rotamase) (Azide free) (HRP); Rotamase; anti-FKBP14 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-FKBP14 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: FKBP14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 143-172 amino acids from the C-terminal region of human FKBP14.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(FKBP14 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the FKBP14 antibody detected the FKBP14 protein (arrow).)

Related Product Information for anti-FKBP14 antibody

PPIases accelerate the folding of proteins during protein synthesis.

Product Categories/Family for anti-FKBP14 antibody

Antibodies; Binding Proteins

References

Zhang, Z., et al. Protein Sci. 13(10):2819-2824(2004). Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003). Patterson, C.E., et al. Genomics 79(6):881-889(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 8923659
• NCBI GeneID: 55033
• NCBI Accession #: NP_060416.1
• NCBI GenBank Nucleotide #: NM_017946.3
• UniProt Accession #: Q9NWM8
• Molecular Weight: 24,172 Da
• NCBI Official Full Name: peptidyl-prolyl cis-trans isomerase FKBP14
• NCBI Official Synonym Full Names: FK506 binding protein 14, 22 kDa
• NCBI Official Symbol: FKBP14
• NCBI Official Synonym Symbols: EDSKMH; FKBP22; IPBP12
• NCBI Protein Information: peptidyl-prolyl cis-trans isomerase FKBP14; 22 kDa FK506-binding protein; 22 kDa FKBP; FK506-binding protein 14; FKBP-22; PPIase FKBP14; rotamase
• UniProt Protein Name: Peptidyl-prolyl cis-trans isomerase FKBP14
• Protein Family: Peptidyl-prolyl cis-trans isomerase
• UniProt Gene Name: FKBP14
• UniProt Synonym Gene Names: FKBP22; PPIase FKBP14; 22 kDa FKBP; FKBP-22; FKBP-14
• UniProt Entry Name: FKB14_HUMAN

NCBI Description

The protein encoded by this gene is a member of the FK506-binding protein family of peptidyl-prolyl cis-trans isomerases. The encoded protein is found in the lumen of the endoplasmic reticulum, where it is thought to accelerate protein folding. Defects in this gene are a cause of a type of Ehlers-Danlos syndrome (EDS). Both a protein-coding variant and noncoding variants are transcribed from this gene. [provided by RefSeq, Mar 2012]

Uniprot Description

FKBP14: PPIases accelerate the folding of proteins during protein synthesis. Defects in FKBP14 are the cause of Ehlers-Danlos syndrome with progressive kyphoscoliosis, myopathy, and hearing loss (EDSKMH). A syndrome with features of Ehlers-Danlos syndrome types VIA and VIB on the one hand, and the collagen VI- related congenital myopathies Ullrich congenital muscular dystrophy and Bethlem myopathy on the other hand. Clinically, this disorder is characterized by the following features: severe generalized hypotonia at birth with marked muscle weakness that improve in infancy; early-onset progressive kyphoscoliosis; joint hypermobility without contractures; hyperelastic skin with follicular hyperkeratosis, easy bruising, and occasional abnormal scarring; myopathy as confirmed by muscle MRI, histology, and electron microscopy; hearing impairment, which is predominantly sensorineural; and a normal ratio of lysyl pyridinoline to hydroxylysyl pyridinoline (LP/HP) in urine.

Protein type: Secreted, signal peptide; Secreted; Isomerase; EC 5.2.1.8

Chromosomal Location of Human Ortholog: 7p14.3

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum lumen

Molecular Function: FK506 binding; peptidyl-prolyl cis-trans isomerase activity; calcium ion binding

Biological Process: protein peptidyl-prolyl isomerization; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; unfolded protein response

Disease: Ehlers-danlos Syndrome With Progressive Kyphoscoliosis, Myopathy, And Hearing Loss