Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FKBP14 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate The cell lysates (20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FKBP14 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : 293T cell lysate Lane 2. : FKBP14 Transfected 293T cell lysate )
Mouse anti-Human FKBP14 Monoclonal Antibody | anti-FKBP14 antibody
FKBP14 antibody
Gene Names
FKBP14; EDSKMH; FKBP22; IPBP12; EDSKSCL2
Reactivity
Human
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence
Purity
By protein-A affinity chromatography
Synonyms
FKBP14; Monoclonal Antibody; FKBP14 antibody; Peptidyl-prolyl cis-trans isomerase FKBP14; FKBP22; 22 kDa FK506 binding protein; FK506 binding protein 14 (22 kDa); FK506 binding protein 14; FKBP 22; Peptidyl prolyl cis trans isomerase; PPIase; Rotamase; anti-FKBP14 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
AT18E2
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
211
Applicable Applications for anti-FKBP14 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
Antigen Species
Human
Immunogen
Recombinant human FKBP14 (20-211aa) purified from E. coli
Clone Note
Anti-human FKBP14 mAb, clone AT18E2, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human FKBP14 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FKBP14 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate The cell lysates (20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FKBP14 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : 293T cell lysate Lane 2. : FKBP14 Transfected 293T cell lysate )
Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FKBP14 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate The cell lysates (20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FKBP14 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : 293T cell lysate Lane 2. : FKBP14 Transfected 293T cell lysate )
Immunofluorescence (IF)
(ICC/IF analysis of FKBP14 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human FKBP14 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Immunofluorescence (IF)
(ICC/IF analysis of FKBP14 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human FKBP14 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Related Product Information for anti-FKBP14 antibody
FKBP14, also known as 22 kDa FK506-binding protein, is an enzyme that accelerates the folding of proteins during protein synthesis. This protein contains two EF-hand domains and one PPIase FKBP-type domain. Truncation of the amino-terminus of FKBP14 greatly reduces peptidyl prolyl cis-trans isomerase activity, therefore suggesting that the PPIase FKBP-type domain must be located at the N-terminus.
Product Categories/Family for anti-FKBP14 antibody
References
Tremmel D., et al. (2007) J Mol Biol. 369 (1): 55-68. ; Budiman C., et al. (2009) FEBS J. 276 (15): 4091-101.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
24,172 Da
NCBI Official Full Name
peptidyl-prolyl cis-trans isomerase FKBP14
NCBI Official Synonym Full Names
FK506 binding protein 14
NCBI Official Symbol
FKBP14
NCBI Official Synonym Symbols
EDSKMH; FKBP22; IPBP12; EDSKSCL2
NCBI Protein Information
peptidyl-prolyl cis-trans isomerase FKBP14
UniProt Protein Name
Peptidyl-prolyl cis-trans isomerase FKBP14
Protein Family
UniProt Gene Name
FKBP14
UniProt Synonym Gene Names
FKBP22; PPIase FKBP14; 22 kDa FKBP; FKBP-22; FKBP-14
NCBI Description
The protein encoded by this gene is a member of the FK506-binding protein family of peptidyl-prolyl cis-trans isomerases. The encoded protein is found in the lumen of the endoplasmic reticulum, where it is thought to accelerate protein folding. Defects in this gene are a cause of a type of Ehlers-Danlos syndrome (EDS). Both a protein-coding variant and noncoding variants are transcribed from this gene. [provided by RefSeq, Mar 2012]
Uniprot Description
PPIases accelerate the folding of proteins during protein synthesis.
Research Articles on FKBP14
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Product Notes
The FKBP14 fkbp14 (Catalog #AAA200442) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The FKBP14 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FKBP14 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF). Researchers should empirically determine the suitability of the FKBP14 fkbp14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FKBP14, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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