CAT active protein

CAT, 1-527aa, Human, His tag, E Coli (Bioactivity Validated)

Cat. Num. AAA204136

• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: CAT; 1-527aa; Human; His tag; E Coli (Bioactivity Validated); Catalase; CAT active protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MADSRDPASD QMQHWKEQRA AQKADVLTTG AGNPVGDKLN VITVGPRGPL LVQDVVFTDE MAHFDRERIP ERVVHAKGAG AFGYFEVTHD ITKYSKAKVF EHIGKKTPIA VRFSTVAGES GSADTVRDPR GFAVKFYTED GNWDLVGNNT PIFFIRDPIL FPSFIHSQKR NPQTHLKDPD MVWDFWSLRP ESLHQVSFLF SDRGIPDGHR HMNGYGSHTF KLVNANGEAV YCKFHYKTDQ GIKNLSVEDA ARLSQEDPDY GIRDLFNAIA TGKYPSWTFY IQVMTFNQAE TFPFNPFDLT KVWPHKDYPL IPVGKLVLNR NPVNYFAEVE QIAFDPSNMP PGIEASPDKM LQGRLFAYPD THRHRLGPNY LHIPVNCPYR ARVANYQRDG PMCMQDNQGG APNYYPNSFG APEQQPSALE HSIQYSGEVR RFNTANDDNV TQVRAFYVNV LNEEQRKRLC ENIAGHLKDA QIFIQKKAVK NFTEVHPDYG SHIQALLDKY NAEKPKNAIH TFVQSGSHLA AREKANL
• Sequence Length: 527

• Applicable Applications for CAT active protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Biological Activity: Specific activity is > 70,000 units/mg. One unit will decompose 1.0umole of H2O2 per minute at pH 8.0 at 25C.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for CAT active protein

CAT is a key antioxidant enzyme in the bodies defense against oxidative stress. Also, it is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. CAT converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Recombinant human CAT protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for CAT active protein

Metabolism

References

Freitas MO, et al. (2011) J Biol Chem. 2011 Nov 25;286 (47):40509-19. Epub 2011 Oct 5.

NCBI and Uniprot Product Information

• NCBI GI #: 4557014
• NCBI GeneID: 847
• NCBI Accession #: NP_001743
• NCBI GenBank Nucleotide #: NM_001752.3
• UniProt Accession #: P04040; Q2M1U4; Q4VXX5; Q9BWT9; Q9UC85; A8K6C0; B2RCZ9; D3DR07
• Molecular Weight: 61.9 kDa (547aa)
• NCBI Official Full Name: catalase
• NCBI Official Synonym Full Names: catalase
• NCBI Official Symbol: CAT
• NCBI Protein Information: catalase
• UniProt Protein Name: Catalase
• Protein Family: Catenin
• UniProt Gene Name: CAT

NCBI Description

This gene encodes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene. [provided by RefSeq, Oct 2009]

Uniprot Description

Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.