Chicken Cat (Catalase) ELISA Kit | Cat elisa kit

Chicken Cat (Catalase) ELISA Kit

Cat. Num. AAA7606837

• Reactivity: Chicken
• Synonyms: Cat (Catalase); Chicken Cat (Catalase) ELISA Kit; Cat elisa kit

• Reactivity: Chicken
• Sequence Length: 527

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 15.625-1000pg/ml
• Sensitivity: 9.375pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Cat elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Cat antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Cat antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Cat amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Cat can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4557014
• NCBI GeneID: 847
• NCBI Accession #: NP_001743.1
• NCBI GenBank Nucleotide #: NM_001752.3
• UniProt Accession #: P04040; Q2M1U4; Q4VXX5; Q9BWT9; Q9UC85; A8K6C0; B2RCZ9; D3DR07
• Molecular Weight: 59,756 Da
• NCBI Official Full Name: catalase
• NCBI Official Synonym Full Names: catalase
• NCBI Official Symbol: CAT
• NCBI Protein Information: catalase
• UniProt Protein Name: Catalase
• UniProt Gene Name: CAT

NCBI Description

This gene encodes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene. [provided by RefSeq, Oct 2009]

Uniprot Description

Catalase: Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. Homotetramer. Belongs to the catalase family.

Protein type: Amino Acid Metabolism - tryptophan; Apoptosis; EC 1.11.1.6; Endoplasmic reticulum; Energy Metabolism - methane; Hydrolase; Mitochondrial; Oxidoreductase

Chromosomal Location of Human Ortholog: 11p13

Cellular Component: extracellular region; focal adhesion; intracellular membrane-bound organelle; membrane; mitochondrion; peroxisomal matrix; peroxisomal membrane; peroxisome

Molecular Function: antioxidant activity; catalase activity; enzyme binding; heme binding; NADP binding; oxidoreductase activity, acting on peroxide as acceptor; protein homodimerization activity; receptor binding

Biological Process: hydrogen peroxide catabolic process; negative regulation of apoptosis; neutrophil degranulation; osteoblast differentiation; protein homotetramerization; protein tetramerization; purine nucleotide catabolic process; response to hydrogen peroxide; response to reactive oxygen species; UV protection

Disease: Acatalasemia