SDS-Page
(SDS-PAGE)
Coagulation Factor VII (F7) Recombinant Protein | F7 recombinant protein
Recombinant Coagulation Factor VII (F7)
Applications
SDS-Page, Western Blot
Purity
Greater than 80% by SDS-PAGE
Synonyms
Coagulation Factor VII (F7); Recombinant Coagulation Factor VII (F7); F7/Coagulation Factor VII; FVII; SPCA; proconvertin; Cothromboplastin; Serum Prothrombin Conversation Accelerator; Stable Factor; Eptacog alfa; F7 recombinant protein
Host
E Coli
Purity/Purification
Greater than 80% by SDS-PAGE
Form/Format
Freeze-dried powder
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Sequence
Ile194~Leu446
Applicable Applications for F7 recombinant protein
Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
Endotoxin
<1.0EU per 1ug (determined by the LAL method)
Tag
N-terminal His and GST Tag
Expression System
Prokaryotic expression
Organism Species
Rat
Isoelectric Point
7.4
Subcellular Location
Secreted
Reconstitution
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.
Preparation and Storage
Store at 2-8 degree C for one month, -80 degree C for 12 months
Stable for 12 months if correctly stored.
The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.
Stable for 12 months if correctly stored.
The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.
SDS-Page
(SDS-PAGE)
SDS-Page
(SDS-PAGE)
Product Categories/Family for F7 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
50,399 Da
NCBI Official Full Name
coagulation factor VII
NCBI Official Synonym Full Names
coagulation factor VII
NCBI Official Symbol
F7
NCBI Protein Information
coagulation factor VII
UniProt Protein Name
Coagulation factor VII
Protein Family
UniProt Gene Name
F7
UniProt Entry Name
FA7_RAT
Uniprot Description
Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium ().
Research Articles on F7
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Product Notes
The F7 f7 (Catalog #AAA2125510) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Coagulation Factor VII (F7) can be used in a range of immunoassay formats including, but not limited to, Positive Control, Immunogen, SDS-PAGE, Western Blot (WB). Researchers should empirically determine the suitability of the F7 f7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Ile194~Leu 446. It is sometimes possible for the material contained within the vial of "Coagulation Factor VII (F7), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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