Caspase 3, active Active Protein | CASP3 active protein

Caspase 3, active, Recombinant, Human (CPP-32, Apoptain, Yama, SCA-1)

Cat. Num. AAA636958

• Gene Names: CASP3; CPP32; SCA-1; CPP32B
• Purity: Molecular Biology Grade
• Synonyms: Caspase 3; active; Recombinant; Human (CPP-32; Apoptain; Yama; SCA-1); CASP3 active protein

• Host: E Coli
• Purity/Purification: Molecular Biology Grade
• Form/Format: Supplied as a lyophilized powder. Reconstitute with sterile PBS, 15% glycerol to 1unit/ml.

• Application Notes: Suitable for use in Caspase 3 activity assays, studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase assays. Other applications not have not been tested.; Dilution: Caspase-3 activity assay: Use 0.5-1unit per test for fluorometric caspase assay. Use 2-5 units per test for colorimetric assay.
• Specific Activity: 300,000-400,000 units/mg; ; Unit Definition:; One unit of the recombinant caspase-3 is the enzyme activity that cleaves 1nmol of the caspase substrate DEVD-pNA (pNA: pnitroanaline) per hour at 37 degree C in a reaction solution containing 50mM Hepes, pH 7.2, 50mM sodium chloride, 0.1% Chaps, 10mM EDTA, 10mM DTT, 5% glycerol.
• Preparation and Storage: For long-term storage, store at -70 degree C. Lyophilized product is stable for 12 months at -70 degree C. Reconstitute with sterile PBS, 15 % glycerol. Reconstituted product is stable for 3-6 months at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for CASP3 active protein

Caspase-3 (also know as CPP32, Yama and apopain) is a member of the interleukin-1b converting enzyme (ICE) family of cysteine proteases. Caspase-3 exists in cells as an inactive 32kD proenzyme. During apoptosis procaspase-3 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases, such as caspase-6 (Mch2), caspase-8 (Flice) and grazyme B. The processed form of caspase-3 consists of large (17 kD) and small (11 kD) subunits which associate to form the active enzyme. The active caspase-3 has been shown involving in the proteolysis of several important molecules, such as poly (ADP-ribose) polymerase (PARP), the sterol regulatory element binding proteins (SREBPs), focal adhesion kinase (FAK), and others. The active recombinant caspase-3 is routinely tested for its ability to enzymatically cleave the caspase-3 colorimetric substrate Ac-DEVD-pNA.

The active recombinant human caspase-3 was expressed in E. coli. The expressed caspase-3 spontaneously undergoes autoprocessing to yield the subunits characteristic of the native enzyme. The recombinant caspase-3 preferentially cleaves caspase-3 substrates (e.g., DEVD-AFC or DEVD-pNA). In combination with caspase-3 activity assays, the active recombinant caspase-3 is very useful in screening of caspase inhibitors for pharmaceutical applications. The recombinant enzyme can also be used as a positive control in caspase assays or in determining the specificity of caspase substrates.
For a complete caspase-3 assay protocol, please refer to Caspase-3/CPP32 Fluorometric or Colorimetric Assay Kits (MyBioSource# C2087-10 and C2087-20).

Product Categories/Family for CASP3 active protein

Molecular Biology; MB-Apoptosis; Caspase

NCBI and Uniprot Product Information

• NCBI GI #: 14790119
• NCBI GeneID: 836
• NCBI Accession #: NP_004337.2
• NCBI GenBank Nucleotide #: NM_004346.3
• UniProt Accession #: P42574; Q96AN1; Q96KP2; A8K5M2; D3DP53
• Molecular Weight: 31,608 Da
• NCBI Official Full Name: caspase-3 preproprotein
• NCBI Official Synonym Full Names: caspase 3, apoptosis-related cysteine peptidase
• NCBI Official Symbol: CASP3
• NCBI Official Synonym Symbols: CPP32; SCA-1; CPP32B
• NCBI Protein Information: caspase-3; CASP-3; CPP-32; apopain; procaspase3; protein Yama; PARP cleavage protease; cysteine protease CPP32; SREBP cleavage activity 1; caspase 3, apoptosis-related cysteine protease
• UniProt Protein Name: Caspase-3
• Protein Family: Caspase
• UniProt Gene Name: CASP3
• UniProt Synonym Gene Names: CPP32; CASP-3; CPP-32; SCA-1
• UniProt Entry Name: CASP3_HUMAN

NCBI Description

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

CASP3: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce. Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. Inhibited by isatin sulfonamides. Belongs to the peptidase C14A family.

Protein type: Motility/polarity/chemotaxis; Apoptosis; Protease; EC 3.4.22.56

Chromosomal Location of Human Ortholog: 4q34

Cellular Component: nucleoplasm; plasma membrane; cytosol; nucleus

Molecular Function: cyclin-dependent protein kinase inhibitor activity; peptidase activity; protein binding; cysteine-type endopeptidase activity; aspartic-type endopeptidase activity

Biological Process: extracellular matrix organization and biogenesis; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; apoptosis; heart development; negative regulation of activated T cell proliferation; negative regulation of B cell proliferation; proteolysis; regulation of caspase activity; neuron differentiation; extracellular matrix disassembly; sensory perception of sound; B cell homeostasis; positive regulation of neuron apoptosis; response to wounding; erythrocyte differentiation; T cell homeostasis; DNA fragmentation during apoptosis; response to UV; cell structure disassembly during apoptosis; release of cytochrome c from mitochondria; cell fate commitment; negative regulation of cyclin-dependent protein kinase activity; keratinocyte differentiation; neuron apoptosis; induction of apoptosis via death domain receptors; platelet formation; caspase activation via cytochrome c; induction of apoptosis by oxidative stress; response to DNA damage stimulus; negative regulation of apoptosis