Human Caspase 3 PicoKine ELISA Kit | CASP3 elisa kit

Human Caspase 3 PicoKine ELISA Kit

Cat. Num. AAA177596

• Gene Names: CASP3; CPP32; SCA-1; CPP32B
• Reactivity: Human
• Synonyms: Caspase 3 PicoKine; Human Caspase 3 PicoKine ELISA Kit; Caspase-3; CASP-3; CASP3; CASP 3; Caspase3; Caspase 3; SCA-1; SCA 1; SCA1 ; caspase 3; CASP3 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human Caspase 3
• Sequence Length: 277

• Assay Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <10pg/ml
• Sample: For quantitative detection of human Caspase 3 in cell culture supernates, tissue homogenates and serum.
• Immunogen: E Coli,S29-D175&A183-H277
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml human Caspase 3 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, tissue homogenates or serum to each empty well. It is recommended that each human Caspase 3 standard solution and each sample be measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(pg/ml)|140|517|1437|168|724|1583; Standard deviation|4.9|21.71|70.41|8.74|47.06|115.56; CV(%)|3.5|4.2|4.9|5.2|6.5|7.3
• Sample Data: Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000; O.D.|0.020|0.069|0.114|0.228|0.443|0.783|1.395|2.220
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Human Caspase 3 PicoKine ELISA Kit standard curve )

Related Product Information for CASP3 elisa kit

Principle of the assay: Human Caspase 3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Caspase 3 has been precoated onto 96-well plates. Standards(Expression system for standard: E Coli, Immunogen sequence: S29-D175 & A183-H277) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Caspase 3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Caspase 3 amount of sample captured in plate.

Background: Caspase-3 is a caspase protein that is encoded by the CASP3 gene. This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

NCBI and Uniprot Product Information

• NCBI GI #: 14790119
• NCBI GeneID: 836
• NCBI Accession #: NP_004337.2
• NCBI GenBank Nucleotide #: NM_004346.3
• UniProt Accession #: P42574; Q96AN1; Q96KP2; A8K5M2; D3DP53
• Molecular Weight: 31,608 Da
• NCBI Official Full Name: caspase-3 preproprotein
• NCBI Official Synonym Full Names: caspase 3
• NCBI Official Symbol: CASP3
• NCBI Official Synonym Symbols: CPP32; SCA-1; CPP32B
• NCBI Protein Information: caspase-3
• UniProt Protein Name: Caspase-3
• Protein Family: Caspase
• UniProt Gene Name: CASP3
• UniProt Synonym Gene Names: CPP32; CASP-3; CPP-32; SCA-1
• UniProt Entry Name: CASP3_HUMAN

NCBI Description

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

CASP3: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce. Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. Inhibited by isatin sulfonamides. Belongs to the peptidase C14A family.

Protein type: Protease; Apoptosis; EC 3.4.22.56; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 4q34

Cellular Component: cytosol; lipid raft; nucleoplasm; nucleus; plasma membrane

Molecular Function: aspartic-type endopeptidase activity; caspase activator activity; cyclin-dependent protein kinase inhibitor activity; cysteine-type endopeptidase activity; death receptor binding; peptidase activity; phospholipase A2 activator activity; protease binding; protein binding; protein complex binding

Biological Process: apoptosis; B cell homeostasis; caspase activation; caspase activation via cytochrome c; cell fate commitment; cell structure disassembly during apoptosis; DNA fragmentation during apoptosis; erythrocyte differentiation; heart development; hippocampus development; keratinocyte differentiation; learning and/or memory; negative regulation of activated T cell proliferation; negative regulation of apoptosis; negative regulation of B cell proliferation; negative regulation of cyclin-dependent protein kinase activity; nerve growth factor receptor signaling pathway; neuron apoptosis; neuron differentiation; platelet formation; positive regulation of neuron apoptosis; proteolysis; regulation of macroautophagy; response to amino acid stimulus; response to antibiotic; response to cobalt ion; response to DNA damage stimulus; response to drug; response to estradiol stimulus; response to glucocorticoid stimulus; response to glucose stimulus; response to hydrogen peroxide; response to hypoxia; response to lipopolysaccharide; response to nicotine; response to UV; response to X-ray; sensory perception of sound; T cell homeostasis; wound healing