Rabbit anti-Human FABP4 Polyclonal Antibody | anti-FABP4 antibody

Anti-FABP4 Antibody, Rabbit Polyclonal

Cat. Num. AAA8106187

• Gene Names: FABP4; aP2; ALBP; AFABP; A-FABP; HEL-S-104
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: FABP4; Polyclonal Antibody; Anti-FABP4 Antibody; Rabbit Polyclonal; FABP4/ALBP/A-FABP Antibody; Rabbit PAb; Antigen Affinity Purified; fatty acid binding protein 4; adipocyte; Anti-A-FABP Antibody; Anti-AFABP Antibody; Anti-ALBP Antibody; Anti-aP2 Antibody; Anti-HEL-S-104 Antibody; anti-FABP4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human FABP4/ALBP/A-FABP
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-FABP4 antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IP: 1-4uL/mg of lysate
• Immunogen: Recombinant Human FABP4/ALBP/A-FABP protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human FABP4/ALBP/A-FABP (rh FABP4/ALBP/A-FABP). FABP4/ALBP/A-FABP specific IgG was purified by Human FABP4/ALBP/A-FABP affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(FABP4 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg Hela Whole Cell Lysate2 uL anti-FABP4 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-FABP4 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 15 kDaObserved band size: 15 kDa)

Western Blot (WB)

(Anti-FABP4 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:15 kDaObserved band size:16 kDa)

Related Product Information for anti-FABP4 antibody

Fatty acid-binding protein, adipocyte, also known as Adipocyte-type fatty acid-binding protein, Fatty acid-binding protein 4, Adipocyte lipid-binding protein, and FABP4, is a cytoplasm protein which belongs to thecalycin superfamily and Fatty-acid binding protein (FABP) family. In familial combined hyperlipidemia (FCHL), FABP4 correlated to body mass index (BMI), waist circumference and homeostasis model assessment (HOMA) index.FABP4 levels were associated with triglyceride-rich lipoproteins. In humans serum FABP4 levels correlate significantly with features of PCOS. It appears to be a determinant of atherogenic dyslipidemia. FABP4 pathway mediates the sebaceous gland hyperplasia in keratinocyte-specific Pten-null mice. FABP4 concentration significantly increased with an increasing of MS features and was strongly correlated with body-mass index, triglycerides, HDL-cholesterol concentrations and blood pressure. Patients in the highest quartile of FABP4 presented a six-fold increased odds ratio for MS and a three-fold increased odds for LD, adjusted by age, sex, body-mass index and the antiretroviral therapy. FABP4 is a strong plasma marker of metabolic disturbances in HIV-infected patients, and therefore, could serve to guide therapeutic intervention in this group of patients.

Product Categories/Family for anti-FABP4 antibody

Early Mesodermal Lineage Markers

References

van Dongen, M.J. et al., 2002, J Am Chem Soc. 124 (40): 11874-80. Coll, B. et al., 2008, Atherosclerosis 199 (1):147-53. Hoashi, S. et al., 2008, BMC Genet. 9 :84. Cai, H. et al., 2010, Bioorg Med Chem Lett 20 (12):3675-9.

NCBI and Uniprot Product Information

• NCBI GI #: 119781
• NCBI GeneID: 2167
• UniProt Accession #: P15090; Q6IBA1
• Molecular Weight: 132
• NCBI Official Full Name: Fatty acid-binding protein, adipocyte
• NCBI Official Synonym Full Names: fatty acid binding protein 4, adipocyte
• NCBI Official Symbol: FABP4
• NCBI Official Synonym Symbols: aP2; ALBP; AFABP; A-FABP; HEL-S-104
• NCBI Protein Information: fatty acid-binding protein, adipocyte; fatty acid-binding protein 4; adipocyte lipid-binding protein; epididymis secretory protein Li 104; adipocyte-type fatty acid-binding protein
• UniProt Protein Name: Fatty acid-binding protein, adipocyte
• Protein Family: Fatty acid-binding protein
• UniProt Gene Name: FABP4
• UniProt Synonym Gene Names: ALBP; A-FABP; AFABP
• UniProt Entry Name: FABP4_HUMAN

NCBI Description

FABP4 encodes the fatty acid binding protein found in adipocytes. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism. [provided by RefSeq, Jul 2008]

Uniprot Description

FABP4: a lipid transport protein that is the predominant fatty acid binding protein found in adipocytes. Also expressed in macrophages. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus. Homodimer. Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. Interacts with PPARG. Monomer. FABP4 knockout mice fed a high-fat and high-calorie diet become obese but develop neither insulin resistance nor diabetes, suggesting that this protein might be a link between obesity and insulin resistance and diabetes. Mice deficient in both FABP4 and ApoE show protection against atherosclerosis when compared with mice deficient only in ApoE. Mice carrying a FABP4 genetic variant exhibit both reduced FABP4 expression and a reduced potential for developing type 2 diabetes and coronary heart disease. A related study in humans indicated a similar pattern, suggesting that FABP4 may be a potential therapeutic target in the treatment of these disorders. Belongs to the calycin superfamily,fatty-acid binding protein (FABP) family.

Protein type: Lipid-binding

Chromosomal Location of Human Ortholog: 8q21

Cellular Component: cytoplasm; lipid particle; nucleus

Molecular Function: transporter activity; fatty acid binding

Biological Process: cholesterol homeostasis; transport; white fat cell differentiation; triacylglycerol catabolic process; brown fat cell differentiation; negative regulation of protein kinase activity; cytokine production; negative regulation of transcription, DNA-dependent; positive regulation of inflammatory response