BMP-4 active protein

Human BMP-4 (E Coli)

Cat. Num. AAA696015

• Gene Names: BMP4; ZYME; BMP2B; OFC11; BMP2B1; MCOPS6
• Purity: >=98% as determined by SDS-PAGE and HPLC analyses.
• Synonyms: BMP-4; Human BMP-4 (E Coli); Recombinant Human BMP-4; BMP-4 active protein

• Host: E.coli
• Purity/Purification: >=98% as determined by SDS-PAGE and HPLC analyses.
• Form/Format: Sterile filtered through a 0.2 micron filter. Lyophilized with no additives.
• Sequence: KKNKNCRRHS LYVDFSDVGW NDWIVAPPPGY QAFYCHGDCP FPLADHLNST NHAIVQTLVN SVNSSIPKAC CVPTELSAIS MLYDEYDKV VLKNYQEMVV EGCGCR

• Authenticity: Verified by N-Terminal and Mass Spectrometry analyses(when applicable)
• Endotoxin: Endotoxin level is <0.1 ng/ug of protein(<1EU/ug).
• Protein Content: Verified by UV Spectroscopy and/or SDS-PAGE gel.
• Biological activity: Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected for this effect is ED₅₀ 5-10 ng/mL.
• Reconstitution: Centrifuge the vial prior to opening. Reconstitute in 5mM HCI,pH 3.0 to a concentration of 0.1-1.0 mg/mL. Do not vortex. For extended storage,it is recommended to further dilute ina buffer containing a carrier protein( example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
• Preparation and Storage: Store at -20°C

Testing Data

Related Product Information for BMP-4 active protein

Bone morphogenetic proteins (BMPs) constitute a subfamily within the TGF-beta superfamily of structurally related signaling proteins. Members of this superfamily are widely distributed throughout the body and are involved in diverse physiological processes during both pre- and postnatal life. Like BMP-7, BMP-4 is involved in the development and maintenance of bone and cartilage. Reduced expression of BMP-4 is associated with a number of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. The E Coli derived BMP-4 is a fully active homodimeric protein consisting of two 106 amino acid subunits which correspond to amino acids 303-408 of the full length BMP-4 precursor.

Product Categories/Family for BMP-4 active protein

Stem Cell Research; Apoptosis; Angiogenesis; Bone Biology; Proliferation and Cell Cycle

NCBI and Uniprot Product Information

• NCBI GI #: 115073
• NCBI GeneID: 652
• UniProt Accession #: P12644; Q9UM80
• NCBI Official Full Name: Bone morphogenetic protein 4
• NCBI Official Synonym Full Names: bone morphogenetic protein 4
• NCBI Official Symbol: BMP4
• NCBI Official Synonym Symbols: ZYME; BMP2B; OFC11; BMP2B1; MCOPS6
• NCBI Protein Information: bone morphogenetic protein 4; BMP-4; BMP-2B; bone morphogenetic protein 2B
• UniProt Protein Name: Bone morphogenetic protein 4
• UniProt Gene Name: BMP4
• UniProt Synonym Gene Names: BMP2B; DVR4; BMP-4; BMP-2B
• UniProt Entry Name: BMP4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. This particular family member plays an important role in the onset of endochondral bone formation in humans, and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing in the 5' untranslated region of this gene has been described and three variants are described, all encoding an identical protein. [provided by RefSeq, Jul 2008]

Uniprot Description

BMP4: Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction. Homodimer; disulfide-linked. Interacts with GREM2. Part of a complex consisting of TWSG1 and CHRD. Interacts with the serine proteases, HTRA1 and HTRA3; the interaction with either inhibits BMP4-mediated signaling. The HTRA protease activity is required for this inhibition. Interacts with SOSTDC1. Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines. Belongs to the TGF-beta family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 14q22-q23

Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular region

Molecular Function: heparin binding; protein binding; growth factor activity; cytokine activity; transforming growth factor beta receptor binding; chemoattractant activity

Biological Process: negative regulation of MAP kinase activity; renal system process; extracellular matrix organization and biogenesis; macrophage differentiation; activation of MAPKK activity; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; negative regulation of chondrocyte differentiation; lymphoid progenitor cell differentiation; telencephalon regionalization; germ cell development; post-embryonic development; BMP signaling pathway; regulation of protein import into nucleus; positive regulation of endothelial cell differentiation; positive chemotaxis; chondrocyte differentiation; erythrocyte differentiation; mesonephros development; kidney development; regulation of odontogenesis of dentine-containing teeth; endochondral ossification; negative regulation of immature T cell proliferation in the thymus; positive regulation of cardiac muscle fiber development; specification of organ position; monocyte differentiation; neuron fate commitment; embryonic cranial skeleton morphogenesis; negative regulation of striated muscle development; branching morphogenesis of a tube; negative regulation of mitosis; positive regulation of epidermal cell differentiation; negative regulation of phosphorylation; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; hemopoietic progenitor cell differentiation; positive regulation of endothelial cell proliferation; embryonic digit morphogenesis; negative regulation of transcription, DNA-dependent; alveolus development; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; positive regulation of protein binding; positive regulation of smooth muscle cell proliferation; positive regulation of collagen biosynthetic process; cloacal septation; negative regulation of transcription from RNA polymerase II promoter; embryonic hindlimb morphogenesis; negative regulation of cell cycle; odontogenesis; negative regulation of cell proliferation; smooth muscle development; inner ear receptor cell differentiation; ureteric bud development; intermediate mesodermal cell differentiation; regulation of smooth muscle cell differentiation; positive regulation of BMP signaling pathway; dorsoventral neural tube patterning; negative regulation of epithelial cell proliferation; smoothened signaling pathway; common-partner SMAD protein phosphorylation; negative regulation of T cell differentiation in the thymus; positive regulation of bone mineralization; positive regulation of ossification; odontogenesis of dentine-containing teeth; osteoblast differentiation; positive regulation of osteoblast differentiation; telencephalon development; blood vessel endothelial cell proliferation during sprouting angiogenesis; ureteric bud branching; pituitary gland development; neural tube closure; regulation of cell fate commitment; positive regulation of protein amino acid phosphorylation; negative regulation of myoblast differentiation; positive regulation of neuron differentiation; mesodermal cell fate determination; anterior/posterior axis specification

Disease: Orofacial Cleft 11; Microphthalmia, Syndromic 6