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Sequence Information
Ribonuclease A (RNase A) Active Enzyme | RNase A active enzyme
Active Ribonuclease A (RNase A)
Applications
Cell culture; Activity Assays
Synonyms
Ribonuclease A (RNase A); Active Ribonuclease A (RNase A); RNase A active enzyme
Host
E. coli
Form/Format
10mM Tris, 15mM NaCl, pH 7.5.
Concentration
Original Concentration: 500ug/mL (varies by lot)
Applicable Applications for RNase A active enzyme
Cell culture; Activity Assays
Application Notes
May be suitable for use in other assays to be determined by the end user.
Organism Species
Homo sapiens (Human)
Source
Prokaryotic expression.
Residues
Lys29~Thr156
Tags
N-terminal His-tag
Traits
Liquid
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method).
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8°C for one month.
Aliquot and store at -80°C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 2-8°C for one month.
Aliquot and store at -80°C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Sequence Information
Sequence Information
Gene Sequencing (extract)
Gene Sequencing (extract)
SDS-PAGE
(Sample: Active recombinant RNASE1, Human)
SDS-PAGE
(Sample: Active recombinant RNASE1, Human)
Western Blot (WB)
(Western BlotSample: Recombinant RNASE1, Human;Antibody: Rabbit Anti-Hu RNASE1 Ab (MBS2006402))
Western Blot (WB)
(Western BlotSample: Recombinant RNASE1, Human;Antibody: Rabbit Anti-Hu RNASE1 Ab (MBS2006402))
Testing Data
(Activity: Ribonuclease A (RNASEA) is a member of the pancreatic-type of secretory ribonucleases, a subset of the ribonuclease A superfamily. RNASEA cleaves RNA on the 3' side of pyrimidine nucleotides. The protein acts to degrade ds-RNA over ss-RNA. The activity of recombinant human RNASEA measured by cleaving yeast RNA. One unit of the enzyme causes an increase in absorbance of 0.001 at 260 nm in 15 min when yeast RNA is hydrolyzed at 50°C and pH 5.0. Pipette 50ulof respective recombinant human RNASEA dilution into 100ul 0.1M sodium acetate buffer, pH 5.0, then add 150ul of 0.15mg/ml yeast RNA. The blank tube use 50ul ultrapure water instead of enzyme dilution. All the all tubes Incubate at 50°C for 15 minutes. Read 260 verus blankCalculation)
Testing Data
(Activity: Ribonuclease A (RNASEA) is a member of the pancreatic-type of secretory ribonucleases, a subset of the ribonuclease A superfamily. RNASEA cleaves RNA on the 3' side of pyrimidine nucleotides. The protein acts to degrade ds-RNA over ss-RNA. The activity of recombinant human RNASEA measured by cleaving yeast RNA. One unit of the enzyme causes an increase in absorbance of 0.001 at 260 nm in 15 min when yeast RNA is hydrolyzed at 50°C and pH 5.0. Pipette 50ulof respective recombinant human RNASEA dilution into 100ul 0.1M sodium acetate buffer, pH 5.0, then add 150ul of 0.15mg/ml yeast RNA. The blank tube use 50ul ultrapure water instead of enzyme dilution. All the all tubes Incubate at 50°C for 15 minutes. Read 260 verus blankCalculation)
Similar Products
Product Notes
The RNase A (Catalog #AAA2123999) is an Active Enzyme produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Ribonuclease A (RNase A) can be used in a range of immunoassay formats including, but not limited to, Cell culture; Activity Assays. May be suitable for use in other assays to be determined by the end user. Researchers should empirically determine the suitability of the RNase A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ribonuclease A (RNase A), Active Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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