4-1BB Ligand Active Protein | 4 1BBL active protein

Recombinant Human 4-1BB Ligand

Cat. Num. AAA145001

• Gene Names: TNFSF9; CD137L; 4-1BB-L
• Purity: Greater than 95.0% as determined by SDS-PAGE.
• Synonyms: 4-1BB Ligand; Recombinant Human 4-1BB Ligand; 4 1BBL Human; 4-1BB Ligand Human Recombinant; CD137L; CD137-L; 4-1BBL; TNFSF9; Tumor Necrosis Factor (ligand) Superfamily Member 9; 4 1BBL active protein

• Host: E Coli
• Purity/Purification: Greater than 95.0% as determined by SDS-PAGE.
• Form/Format: Lyophilized from a 0.2?m filtered concentrated solution in PBS, pH 7.4.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: REGPELSPDD PAGLLDLRQG MFAQLVAQNV LLIDGPLSWY SDPGLAGVSL TGGLSYKEDT KELVVAKAGV YYVFFQLELR RVVAGEGSGS VSLALHLQPL RSAAGAAALA LTVDLPPASS EARNSAFGFQ GRLLHLSAGQ RLGVHLHTEA RARHAWQLTQ GATVLGLFRV TPEIPAGLPS PRSE
• Sequence Length: 254

• Solubility: It is recommended to reconstitute the lyophilized 4 1BBL in sterile 18M?-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: Fully biologically active when compared to standard. Determined by the dose-dependent stimulation of IL-8 production by human PBMC. The ED50 for this effect is 5-10ng/ml corresponding to a Specific Activity of 100,000-200,000IU/mg.

Related Product Information for 4 1BBL active protein

Description: 4 1BBL Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 184 amino acids and having a molecular mass of 19.5kDa.The 4 1BBL is purified by proprietary chromatographic techniques.

Introduction: 4-1BBL is a transmembrane cytokine that is part of the tumor necrosis factor (TNF) ligand family. 4-1BBL is a bidirectional signal transducer that performs as a ligand for TNFRSF9, which is a costimulatory receptor molecule in T lymphocytes. TNFSF9 and its TNFRSF9 take part in the antigen presentation development and in the generation of cytotoxic T cells. 4-1BBR is absent from resting T lymphocytes but rapidly expressed upon antigenic stimulation. TNFSF9 reactivates anergic T lymphocytes as well as promoting T lymphocyte proliferation. 4-1BB Ligand is needed for the optimal CD8 responses in CD8 T cells. 4-1BBL is expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interaction. 4-1BBL is expressed by activated B cells, macrophages, dendritic cells, activated T cells, neurons and astrocytes. The interaction of 4-1BB with TNFRSF9 strongly regulates immunity and has been proposed to preferentially control T cell responses based on studies in various murin. 

Product Categories/Family for 4 1BBL active protein

Cytokines and Growth Factors; Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 4507609
• NCBI GeneID: 8744
• NCBI Accession #: NP_003802.1
• NCBI GenBank Nucleotide #: NM_003811.3
• UniProt Accession #: P41273; Q2M3S2
• Molecular Weight: 26,625 Da
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 9
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 9
• NCBI Official Symbol: TNFSF9
• NCBI Official Synonym Symbols: CD137L; 4-1BB-L
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 9; 4-1BB ligand; 4-1BBL; homolog of mouse 4-1BB-L; receptor 4-1BB ligand
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 9
• UniProt Gene Name: TNFSF9
• UniProt Synonym Gene Names: 4-1BBL
• UniProt Entry Name: TNFL9_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This transmembrane cytokine is a bidirectional signal transducer that acts as a ligand for TNFRSF9/4-1BB, which is a costimulatory receptor molecule in T lymphocytes. This cytokine and its receptor are involved in the antigen presentation process and in the generation of cytotoxic T cells. The receptor TNFRSF9/4-1BB is absent from resting T lymphocytes but rapidly expressed upon antigenic stimulation. The ligand encoded by this gene, TNFSF9/4-1BBL, has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine has also been shown to be required for the optimal CD8 responses in CD8 T cells. This cytokine is expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interaction.[provided by RefSeq, Oct 2008]

Uniprot Description

TNFL9: Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages. Belongs to the tumor necrosis factor family.

Protein type: Apoptosis; Cytokine; Membrane protein, integral; Cell cycle regulation

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: extracellular space; plasma membrane; integral to membrane

Molecular Function: cytokine activity; tumor necrosis factor receptor binding; receptor binding

Biological Process: cell proliferation; positive regulation of interferon-gamma production; positive regulation of cytotoxic T cell differentiation; cell-cell signaling; positive regulation of interleukin-12 production; apoptosis; positive regulation of interleukin-6 production; immune response; myeloid dendritic cell differentiation; signal transduction; positive regulation of activated T cell proliferation