Testing Data
(Mouse brain lysate was probed with MBS633464 (0.5ug/ml). Proteins were visualized using an IgG (HRP) goat anti-rat secondary antibody and using a chemiluminescence detection system. Arrow indicates ZO-1 (~220kD).)
Rat anti-Mouse ZO-1 Monoclonal Antibody | anti-ZO-1 antibody
ZO-1 (Tight Junction-associated Polypeptide, Zonula Occludens 1) (FITC)
Testing Data
(Mouse brain lysate was probed with MBS633464 (0.5ug/ml). Proteins were visualized using an IgG (HRP) goat anti-rat secondary antibody and using a chemiluminescence detection system. Arrow indicates ZO-1 (~220kD).)
Testing Data
(Mouse brain lysate was probed with MBS633464 (0.5ug/ml). Proteins were visualized using an IgG (HRP) goat anti-rat secondary antibody and using a chemiluminescence detection system. Arrow indicates ZO-1 (~220kD).)
NCBI and Uniprot Product Information
Uniprot Description
Function: The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells
By similarity.
Subunit structure: Homodimer, and heterodimer with TJP2/ZO-2 and TJP3/ZO-3. Interacts with CGN/cingulin, CALM, CXADR, GJD3, HSPA4 and UBN1. Interacts (via ZU5 domain) with CDC42BPB. Interacts (via PDZ domain) with GJA1
By similarity. Interacts with OCLN, claudins, GJA12 and KIRREL1. Interacts with BVES (via the C-terminus cytoplasmic tail). Interacts (via ZU5 domain) with MYZAP. Interacts (via PDZ domains) with ANKRD2
By similarity. Ref.3 Ref.4 Ref.5 Ref.12
Subcellular location: Cell membrane; Peripheral membrane protein; Cytoplasmic side
By similarity. Cell junction › tight junction
By similarity. Cell junction › gap junction. Cytoplasm › myofibril › sarcomere › I band. Note: Moves from the cytoplasm to the cell membrane concurrently with cell-cell contact. Detected at the leading edge of migrating and wounded cells
By similarity. Ref.12
Domain: The second PDZ domain mediates interaction with GJA12. Ref.4
Post-translational modification: Phosphorylated. Dephosphorylated by PTPRJ
By similarity.
Sequence similarities: Belongs to the MAGUK family.Contains 1 guanylate kinase-like domain.Contains 3 PDZ (DHR) domains.Contains 1 SH3 domain.Contains 1 ZU5 domain.
Research Articles on ZO-1
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Product Notes
The ZO-1 tjp1 (Catalog #AAA6243564) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The ZO-1 (Tight Junction-associated Polypeptide, Zonula Occludens 1) (FITC) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ZO-1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB). Researchers should empirically determine the suitability of the ZO-1 tjp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZO-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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