Rabbit anti-Human REC8 Polyclonal Antibody | anti-REC8 antibody

REC8, ID (REC8, REC8L1, Meiotic recombination protein REC8 homolog, Cohesin Rec8p) (Azide free) (HRP)

Cat. Num. AAA6341173

• Gene Names: REC8; Rec8p; REC8L1; HR21spB
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: REC8; Polyclonal Antibody; ID (REC8; REC8L1; Meiotic recombination protein REC8 homolog; Cohesin Rec8p) (Azide free) (HRP); Cohesin Rec8p; anti-REC8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 547

• Applicable Applications for anti-REC8 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: REC8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 193-222 amino acids from the Central region of human REC8.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(REC8 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the REC8 antibody detected the REC8 protein (arrow).)

Related Product Information for anti-REC8 antibody

This gene encodes a member of the kleisin family of SMC (structural maintenance of chromosome) protein partners. The protein localizes to the axial elements of chromosomes during meiosis in both oocytes and spermatocytes. In the mouse, the homologous protein is a key component of the meiotic cohesion complex, which regulates sister chromatid cohesion and recombination between homologous chromosomes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene.

Product Categories/Family for anti-REC8 antibody

Antibodies; Spermatogenesis; Testis Proteins

References

Aston, K.I., et al. Hum. Reprod. 25(6):1383-1397(2010). Erenpreisa, J., et al. Exp. Cell Res. 315(15):2593-2603(2009). Griffin, J., et al. Syst Biol Reprod Med 54(3):163-165(2008). Xu, H., et al. Dev. Cell 8(6):949-961(2005). Prieto, I., et al. Chromosome Res. 12(3):197-213(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 115430229
• NCBI GeneID: 9985
• NCBI Accession #: NP_005123.2; NP_001041670.1
• NCBI GenBank Nucleotide #: NP_005123.2
• UniProt Accession #: O95072
• NCBI Official Full Name: meiotic recombination protein REC8 homolog
• NCBI Official Synonym Full Names: REC8 meiotic recombination protein
• NCBI Official Symbol: REC8
• NCBI Official Synonym Symbols: Rec8p; REC8L1; HR21spB
• NCBI Protein Information: meiotic recombination protein REC8 homolog
• UniProt Protein Name: Meiotic recombination protein REC8 homolog
• Protein Family: Meiotic recombination protein
• UniProt Gene Name: REC8
• UniProt Synonym Gene Names: REC8L1
• UniProt Entry Name: REC8_HUMAN

NCBI Description

This gene encodes a member of the kleisin family of SMC (structural maintenance of chromosome) protein partners. The protein localizes to the axial elements of chromosomes during meiosis in both oocytes and spermatocytes. In the mouse, the homologous protein is a key component of the meiotic cohesion complex, which regulates sister chromatid cohesion and recombination between homologous chromosomes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

REC8: Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II. Belongs to the rad21 family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 14q11.2-q12

Cellular Component: male germ cell nucleus; lateral element; nucleus

Biological Process: meiosis; fertilization; male meiosis I; linear element formation; meiotic recombination; sister chromatid cohesion; spermatogenesis; oocyte maturation; double-strand break repair via homologous recombination; spermatid development