Disulfide Oxidoreductase Recombinant Protein | DsbA recombinant protein
Recombinant Disulfide Oxidoreductase
Gene Names
dsbA; dsf; ECK3852; iarA; JW3832; ppfA
Purity
Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Synonyms
Disulfide Oxidoreductase; Recombinant Disulfide Oxidoreductase; DsbA; Disulfide Oxidoreductase Recombinant; Thiol:disulfide interchange protein dsbA; DsbA recombinant protein
Host
E Coli
Purity/Purification
Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Form/Format
The protein was lyophilized after from a sterile solution containing 50mM sodium phosphate buffer and 100mM sodium chloride.
Sterile Filtered White lyophilized (freeze-dried) powder.
Sterile Filtered White lyophilized (freeze-dried) powder.
Sequence
The sequence of the first five N-terminal amino acidswas determined and was found to be Met-Ly-Lys-Ala-Trp
Sequence Length
208
Solubility
It is recommended to reconstitute the lyophilized DsbA in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Preparation and Storage
Lyophilized DsbA although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution DsbA should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Related Product Information for DsbA recombinant protein
Description: Disulfide Oxidoreductase produced in E Coli is a periplasmic protein isolated from E Coli, containing 208 amino acids having a molecular mass of 23,149 Dalton. The DsbA is purified by proprietary chromatographic techniques.
Introduction: DsbA appears to be necessary for correct formulation of disulfide bonds in exported proteins in vivo. DsbA is useful as a standard in immunoblotting. This protein catalyses the reduction and exchange of disulfide bonds and the oxidation of free sulfhydryl groups in vitro. It is the strongest oxidant of the thioredoxin superfamily. This thio/disulfide oxidoreductase is required for efficient disulfide bond formation in the periplasm of E. coli.
Introduction: DsbA appears to be necessary for correct formulation of disulfide bonds in exported proteins in vivo. DsbA is useful as a standard in immunoblotting. This protein catalyses the reduction and exchange of disulfide bonds and the oxidation of free sulfhydryl groups in vitro. It is the strongest oxidant of the thioredoxin superfamily. This thio/disulfide oxidoreductase is required for efficient disulfide bond formation in the periplasm of E. coli.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,105 Da
NCBI Official Full Name
periplasmic protein disulfide isomerase I
NCBI Official Symbol
dsbA
NCBI Official Synonym Symbols
dsf; ECK3852; iarA; JW3832; ppfA
NCBI Protein Information
periplasmic protein disulfide isomerase I
UniProt Protein Name
Thiol:disulfide interchange protein DsbA
Protein Family
UniProt Gene Name
dsbA
UniProt Synonym Gene Names
dsf; ppfA
UniProt Entry Name
DSBA_ECOLI
NCBI Description
DsbA has a role in cytochrome C synthesis. [More information is available at EcoGene: EG11297].
Uniprot Description
Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by DsbB. Required for pilus biogenesis. PhoP-regulated transcription is redox-sensitive, being activated when the periplasm becomes more reducing (deletion of dsbA/dsbB, treatment with dithiothreitol). MgrB acts between DsbA/DsbB and PhoP/PhoQ in this pathway.
Research Articles on DsbA
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Product Notes
The DsbA dsba (Catalog #AAA142417) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: The sequence of the first five N-terminal amino acidswas determined and was found to be Met-Ly-Lys -Ala-Trp. It is sometimes possible for the material contained within the vial of "Disulfide Oxidoreductase, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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