Monkey Dopamine Receptor D5 ELISA Kit | DRD5 elisa kit

Monkey Dopamine Receptor D5 ELISA Kit

Cat. Num. AAA745156

• Gene Names: DRD5; DBDR; DRD1B; DRD1L2
• Reactivity: Monkey
• Synonyms: Dopamine Receptor D5; Monkey Dopamine Receptor D5 ELISA Kit; DRD5 elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 2.5-50ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This DRD5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey DRD5. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DRD5 elisa kit

Principle of the assay: DRD5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DRD5 antibody and an DRD5-HRP conjugate. The assay sample and buffer are incubated together with DRD5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DRD5 concentration since DRD5 from samples and DRD5-HRP conjugate compete for the anti-DRD5 antibody binding site. Since the number of sites is limited, as more sites are occupied by DRD5 from the sample, fewer sites are left to bind DRD5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DRD5 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for DRD5 elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 181831
• NCBI GeneID: 1816
• Molecular Weight: 52,951 Da
• NCBI Official Full Name: dopamine receptor D5
• NCBI Official Synonym Full Names: dopamine receptor D5
• NCBI Official Symbol: DRD5
• NCBI Official Synonym Symbols: DBDR; DRD1B; DRD1L2
• NCBI Protein Information: D(1B) dopamine receptor; D(1B) dopamine receptor; dopamine D5 receptor; dopamine receptor D1B; d(5) dopamine receptor; D1beta dopamine receptor
• UniProt Protein Name: D(1B) dopamine receptor
• Protein Family: D(1B) dopamine receptor
• UniProt Gene Name: DRD5
• UniProt Synonym Gene Names: DRD1B; DRD1L2
• UniProt Entry Name: DRD5_HUMAN

NCBI Description

This gene encodes the D5 subtype of the dopamine receptor. The D5 subtype is a G-protein coupled receptor which stimulates adenylyl cyclase. This receptor is expressed in neurons in the limbic regions of the brain. It has a 10-fold higher affinity for dopamine than the D1 subtype. Pseudogenes related to this gene reside on chromosomes 1 and 2. [provided by RefSeq, Jul 2008]

Uniprot Description

DRD5: Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase. Defects in DRD5 are a cause of benign essential blepharospasm (BEB). BEB is a primary focal dystonia affecting the orbicularis oculi muscles. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. BEB usually begins in middle age. Initial symptoms include eye irritation and frequent blinking, progressing to involuntary spasms of eyelid closure. Patients have normal eyes. The visual disturbance is due solely to the forced closure of the eyelids. In severe cases, this can lead to functional blindness. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR

Chromosomal Location of Human Ortholog: 4p16.1

Cellular Component: brush border membrane; integral to plasma membrane; plasma membrane

Molecular Function: dopamine receptor activity; dopamine binding; dopamine D1 receptor-like receptor activity

Biological Process: synaptic transmission, dopaminergic; wound healing; adenylate cyclase activation; response to amphetamine; transmission of nerve impulse; norepinephrine-epinephrine vasoconstriction involved in regulation of systemic arterial blood pressure; response to cocaine; G-protein signaling, adenylate cyclase activating pathway; cellular calcium ion homeostasis; synaptic transmission; regulation of female receptivity; regulation of systemic arterial blood pressure by vasopressin; sensitization; negative regulation of blood pressure; dopamine receptor, phospholipase C activating pathway; positive regulation of adenylate cyclase activity; negative regulation of NAD(P)H oxidase activity; associative learning; dopamine receptor, adenylate cyclase activating pathway

Disease: Attention Deficit-hyperactivity Disorder; Blepharospasm, Benign Essential