Human Dopamine (DA) ELISA Kit | DA elisa kit

Human Dopamine (DA) ELISA Kit

Cat. Num. AAA264001

• Gene Names: DRD5; DBDR; DRD1B; DRD1L2
• Reactivity: Human
• Synonyms: Dopamine (DA); Human Dopamine (DA) ELISA Kit; DA elisa kit

• Reactivity: Human
• Sequence Length: 477

• Samples: Human serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 2000 pg/mL-31.2 pg/mL
• Sensitivity: Up to 12 pg/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for DA elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Human DA monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 4503391
• NCBI GeneID: 1816
• NCBI Accession #: NP_000789.1
• NCBI GenBank Nucleotide #: NM_000798.4
• Molecular Weight: 52,951 Da
• NCBI Official Full Name: D(1B) dopamine receptor
• NCBI Official Synonym Full Names: dopamine receptor D5
• NCBI Official Symbol: DRD5
• NCBI Official Synonym Symbols: DBDR; DRD1B; DRD1L2
• NCBI Protein Information: D(1B) dopamine receptor; D(1B) dopamine receptor; dopamine D5 receptor; dopamine receptor D1B; d(5) dopamine receptor; D1beta dopamine receptor
• UniProt Protein Name: D(1B) dopamine receptor
• UniProt Gene Name: DRD5
• UniProt Synonym Gene Names: DRD1B; DRD1L2
• UniProt Entry Name: DRD5_HUMAN

NCBI Description

This gene encodes the D5 subtype of the dopamine receptor. The D5 subtype is a G-protein coupled receptor which stimulates adenylyl cyclase. This receptor is expressed in neurons in the limbic regions of the brain. It has a 10-fold higher affinity for dopamine than the D1 subtype. Pseudogenes related to this gene reside on chromosomes 1 and 2. [provided by RefSeq, Jul 2008]

Uniprot Description

DRD5: Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase. Defects in DRD5 are a cause of benign essential blepharospasm (BEB). BEB is a primary focal dystonia affecting the orbicularis oculi muscles. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. BEB usually begins in middle age. Initial symptoms include eye irritation and frequent blinking, progressing to involuntary spasms of eyelid closure. Patients have normal eyes. The visual disturbance is due solely to the forced closure of the eyelids. In severe cases, this can lead to functional blindness. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass; GPCR, family 1

Chromosomal Location of Human Ortholog: 4p16.1

Cellular Component: brush border membrane; integral to plasma membrane; plasma membrane

Molecular Function: dopamine receptor activity; dopamine binding; dopamine D1 receptor-like receptor activity

Biological Process: synaptic transmission, dopaminergic; wound healing; adenylate cyclase activation; response to amphetamine; transmission of nerve impulse; norepinephrine-epinephrine vasoconstriction involved in regulation of systemic arterial blood pressure; response to cocaine; G-protein signaling, adenylate cyclase activating pathway; cellular calcium ion homeostasis; synaptic transmission; regulation of female receptivity; regulation of systemic arterial blood pressure by vasopressin; sensitization; negative regulation of blood pressure; positive regulation of adenylate cyclase activity; dopamine receptor, phospholipase C activating pathway; negative regulation of NAD(P)H oxidase activity; associative learning; dopamine receptor, adenylate cyclase activating pathway

Disease: Attention Deficit-hyperactivity Disorder; Blepharospasm, Benign Essential