Rabbit anti-Human CCNA2 Polyclonal Antibody | anti-CCNA2 antibody

CCNA2, NT (Cyclin-A2, Cyclin-A, CCN1, CCNA)

Cat. Num. AAA625228

• Gene Names: CCNA2; CCN1; CCNA
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified; Purified by ammonium sulfate precipitation.
• Synonyms: CCNA2; Polyclonal Antibody; NT (Cyclin-A2; Cyclin-A; CCN1; CCNA); Anti -CCNA2; anti-CCNA2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CCNA2.
• Purity/Purification: Purified; Purified by ammonium sulfate precipitation.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-CCNA2 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:50-1:100; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide at the N-term of human CCNA2 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-CCNA2(N-term) Pab in K562 cell line lysates (35ug/lane). CCNA2(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma with Cyclin A (CCNA2) Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of HepG2 cells using Cyclin A (CCNA2) Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CCNA2 antibody

CCNA2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.

Product Categories/Family for anti-CCNA2 antibody

Antibodies; Abs to Cyclin

NCBI and Uniprot Product Information

• NCBI GI #: 4502613
• NCBI GeneID: 890
• NCBI Accession #: NP_001228.1
• NCBI GenBank Nucleotide #: NM_001237.3
• UniProt Accession #: P20248; Q2M3U6; Q4W5P4; Q6LER8; A8K7B6
• Molecular Weight: 48,551 Da
• NCBI Official Full Name: cyclin-A2
• NCBI Official Synonym Full Names: cyclin A2
• NCBI Official Symbol: CCNA2
• NCBI Official Synonym Symbols: CCN1; CCNA
• NCBI Protein Information: cyclin-A2; OTTHUMP00000164087
• UniProt Protein Name: Cyclin-A2
• Protein Family: Cyclin
• UniProt Gene Name: CCNA2
• UniProt Synonym Gene Names: CCN1; CCNA
• UniProt Entry Name: CCNA2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. [provided by RefSeq]

Uniprot Description

CCNA2: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.

Protein type: Cell cycle regulation; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 4q27

Cellular Component: nucleoplasm; male pronucleus; cytoplasm; female pronucleus; nucleus

Molecular Function: protein binding; protein kinase binding

Biological Process: mitosis; organ regeneration; positive regulation of fibroblast proliferation; response to glucagon stimulus; cell division; mitotic cell cycle G2/M transition DNA damage checkpoint; positive regulation of transcription, DNA-dependent; regulation of G2/M transition of mitotic cell cycle; Ras protein signal transduction; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; G2/M transition of mitotic cell cycle; response to estradiol stimulus