Pig Dermatopontin ELISA Kit | DPT elisa kit
Pig Dermatopontin (DPT) ELISA Kit
Reactivity
Pig
Synonyms
Dermatopontin; Pig Dermatopontin (DPT) ELISA Kit; DPT elisa kit
Reactivity
Pig
Specificity
This assay has high sensitivity and excellent specificity for detection of Pig DPT. No significant cross-reactivity or interference between Pig DPT and analogues was observed.
Sequence Length
201
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for DPT elisa kit
Dermatopontin is an extracellular matrix protein with possible functions in cell-matrix interactions and matrix assembly. Dermatopontin is postulated to modify the behavior of TGF-beta through interaction with decorin. Seems to mediate adhesion by cell surface integrin binding. May serve as a communication link between the dermal fibroblast cell surface and its extracellular matrix environment. Enhances TGFB1 activity. Inhibits cell proliferation. Expressed in fibroblasts, heart, skeletal muscle, brain and pancreas. Expressed at an intermediate level in lung and kidney, and at a low level in liver and placenta. Expressed at a lower level in fibroblasts from hypertrophic scar lesional skin and in fibroblasts from patients with systemic sclerosis than in normal skin fibroblasts.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate DPT in samples. An antibody specific for DPT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDPT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DPT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DPT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate DPT in samples. An antibody specific for DPT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDPT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DPT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DPT bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
21,994 Da
NCBI Official Full Name
Dermatopontin
UniProt Protein Name
Dermatopontin
Protein Family
UniProt Gene Name
DPT
UniProt Synonym Gene Names
TRAMP
Uniprot Description
Seems to mediate adhesion by cell surface integrin binding. May serve as a communication link between the dermal fibroblast cell surface and its extracellular matrix environment. Enhances TGFB1 activity. Inhibits cell proliferation (). Accelerates collagen fibril formation, and stabilizes collagen fibrils against low-temperature dissociation.
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Product Notes
The Pig DPT dpt (Catalog #AAA283179) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA283179 ELISA Kit recognizes Pig DPT. It is sometimes possible for the material contained within the vial of "Dermatopontin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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