Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Dermatopontin antibody (DPT-Ab) Standard Curve.)
Mouse Dermatopontin (DPT-Ab) ELISA Kit | DPT-Ab elisa kit
Mouse Dermatopontin Antibody (DPT-Ab) ELISA Kit
Reactivity
Mouse
Synonyms
Dermatopontin (DPT-Ab); Mouse Dermatopontin Antibody (DPT-Ab) ELISA Kit; DPT-Ab; TRAMP; DPT-Ab elisa kit
Reactivity
Mouse
Specificity
Mouse Dermatopontin antibody (DPT-Ab) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DPT-Ab. No significant cross-reactivity or interference between Mouse DPT-Ab and analogues was observed.
Sequence Length
201
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Dermatopontin antibody (DPT-Ab) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Dermatopontin antibody (DPT-Ab) Standard Curve.)
Related Product Information for DPT-Ab elisa kit
Description: This Mouse Dermatopontin antibody (DPT-Ab) ELISA Kit employs a two-site sandwich ELISA to quantitate DPT-Ab in samples. An antibody specific for DPT-Ab has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DPT-Ab present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DPT-Ab is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DPT-Ab bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Dermatopontin is an extracellular matrix protein with possible functions in cell-matrix interactions and matrix assembly. Dermatopontin is postulated to modify the behavior of TGF-beta through interaction with decorin. Seems to mediate adhesion by cell surface integrin binding. May serve as a communication link between the dermal fibroblast cell surface and its extracellular matrix environment. Enhances TGFB1 activity. Inhibits cell proliferation. Expressed in fibroblasts, heart, skeletal muscle, brain and pancreas. Expressed at an intermediate level in lung and kidney, and at a low level in liver and placenta. Expressed at a lower level in fibroblasts from hypertrophic scar lesional skin and in fibroblasts from patients with systemic sclerosis than in normal skin fibroblasts.
Background: Dermatopontin is an extracellular matrix protein with possible functions in cell-matrix interactions and matrix assembly. Dermatopontin is postulated to modify the behavior of TGF-beta through interaction with decorin. Seems to mediate adhesion by cell surface integrin binding. May serve as a communication link between the dermal fibroblast cell surface and its extracellular matrix environment. Enhances TGFB1 activity. Inhibits cell proliferation. Expressed in fibroblasts, heart, skeletal muscle, brain and pancreas. Expressed at an intermediate level in lung and kidney, and at a low level in liver and placenta. Expressed at a lower level in fibroblasts from hypertrophic scar lesional skin and in fibroblasts from patients with systemic sclerosis than in normal skin fibroblasts.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
dermatopontin
Similar Products
Product Notes
The Mouse DPT-Ab (Catalog #AAA9718122) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9718122 ELISA Kit recognizes Mouse DPT-Ab. It is sometimes possible for the material contained within the vial of "Dermatopontin (DPT-Ab), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
