SDS-Page
DOT1L recombinant protein
DOT1L Protein, Human, Recombinant
Gene Names
DOT1L; DOT1; KMT4
Purity
>90% as determined by SDS-PAGE
Synonyms
DOT1L; DOT1L Protein; Human; Recombinant; Human DOT1L/KMT4 Protein; DOT1 Protein; KMT4 Protein; DOT1-like histone H3K79 methyltransferase; DOT1L recombinant protein
Host
E Coli
Purity/Purification
>90% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile 20mM HEPES, 150mM NaCl, 1mM EDTA, 15% glycerol, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Gly2-Lys416
Species
Human
Predicted N Terminal
Gly
Tag
N-cleavage
Protein Construction
A DNA sequence encoding the human DOT1L (NP_115871.1) N-terminal segment (Gly 2-Lys 416) was expressed and purified, with two additional amino acids (Gly & Pro) at the N-terminus.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
SDS-Page
SDS-Page
Related Product Information for DOT1L recombinant protein
Background: Histone-lysine N-methyltransferase, H3 lysine-79 specific, also known as Histone H3-K79 methyltransferase, DOT1-like protein, Lysine N-methyltransferase 4 and DOT1L, is a nucleus protein which belongs to theDOT1 family. In contrast to other lysine histone methyltransferase, DOT1L does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones. DOT1L is an histone methyltransferase. It methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. DOT1L binds to DNA. Methylation of lysine 79 on histone H3 (H3K79) is mediated by DOT1L. It is involved in the regulation of telomeric silencing, development, cell cycle checkpoint and transcription. Mass spectrometry of the DOT1L-containing complex revealed that AF9, ENL and NPM1 were shown to be major DOT1L-interacting proteins. DOT1L might control AF9- and ENL-mediated transcription, regulate RNA processing, and function as a histone chaperone in a NPM1-dependent manner.
References
Nagase T. et al., 2001, DNA Res. 8:85-95. Feng Q.et al., 2002, Curr. Biol. 12:1052-8. Dephoure N. et al., 2008, Proc. Natl. Acad. Sci. USA. 105:10762-7. Gauci S. et al., 2009, Anal. Chem. 81:4493-501. Park,G. et al., 2010, Protein J. 29 (3):213-23.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
184853 MW
NCBI Official Full Name
histone-lysine N-methyltransferase, H3 lysine-79 specific
NCBI Official Synonym Full Names
DOT1 like histone lysine methyltransferase
NCBI Official Symbol
DOT1L
NCBI Official Synonym Symbols
DOT1; KMT4
NCBI Protein Information
histone-lysine N-methyltransferase, H3 lysine-79 specific
UniProt Protein Name
Histone-lysine N-methyltransferase, H3 lysine-79 specific
UniProt Gene Name
DOT1L
UniProt Synonym Gene Names
KIAA1814; KMT4; H3-K79-HMTase
NCBI Description
The protein encoded by this gene is a histone methyltransferase that methylates lysine-79 of histone H3. It is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes. [provided by RefSeq, Aug 2011]
Uniprot Description
Histone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. Binds to DNA.
Research Articles on DOT1L
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Product Notes
The DOT1L dot1l (Catalog #AAA8120849) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Gly2-Lys41 6. It is sometimes possible for the material contained within the vial of "DOT1L, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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