Mouse Cocaine esterase (CES2) ELISA Kit | CES2 elisa kit

Mouse Cocaine esterase (CES2) ELISA Kit

Cat. Num. AAA7243660

• Gene Names: CES2; iCE; CE-2; PCE-2; CES2A1
• Reactivity: Mouse
• Synonyms: Cocaine esterase (CES2); Mouse Cocaine esterase (CES2) ELISA Kit; CES2 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of CES2. No significant cross-reactivity or interference between CES2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CES2 and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CES2 elisa kit

Intended Uses: This CES2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CES2. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: CES2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CES2 antibody and an CES2-HRP conjugate. The assay sample and buffer are incubated together with CES2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CES2 concentration since CES2 from samples and CES2-HRP conjugate compete for the anti-CES2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CES2 from the sample, fewer sites are left to bind CES2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CES2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CES2 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 37622887
• NCBI GeneID: 8824
• NCBI Accession #: NP_932327.1
• NCBI GenBank Nucleotide #: NM_198061.2
• Molecular Weight: 59,959 Da
• NCBI Official Full Name: cocaine esterase isoform 2
• NCBI Official Synonym Full Names: carboxylesterase 2
• NCBI Official Symbol: CES2
• NCBI Official Synonym Symbols: iCE; CE-2; PCE-2; CES2A1
• NCBI Protein Information: cocaine esterase; hCE-2; carboxylesterase 2 (intestine, liver); methylumbelliferyl-acetate deacetylase 2; intestinal carboxylesterase; liver carboxylesterase-2
• UniProt Protein Name: Cocaine esterase
• Protein Family: Liver carboxylesterase
• UniProt Gene Name: CES2
• UniProt Synonym Gene Names: ICE; CE-2; hCE-2
• UniProt Entry Name: EST2_HUMAN

NCBI Description

This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. The protein encoded by this gene is the major intestinal enzyme and functions in intestine drug clearance. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Oct 2010]

Uniprot Description

CES2: Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Shows high catalytic efficiency for hydrolysis of cocaine, 4-methylumbelliferyl acetate, heroin and 6-monoacetylmorphine. Belongs to the type-B carboxylesterase/lipase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Xenobiotic Metabolism - drug metabolism - other enzymes; EC 3.1.1.84; EC 3.1.1.1; EC 3.1.1.56; Endoplasmic reticulum; Hydrolase

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: extracellular space; endoplasmic reticulum; endoplasmic reticulum lumen

Molecular Function: methylumbelliferyl-acetate deacetylase activity

Biological Process: catabolic process