Rabbit anti-Human POLI Polyclonal Antibody | anti-POLI antibody

POLI Antibody

Cat. Num. AAA7103706

• Gene Names: POLI; eta2; RAD30B; RAD3OB
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: POLI; Polyclonal Antibody; POLI Antibody; DNA polymerase iota; Eta2; RAD30 homolog B; RAD30B; anti-POLI antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 740

• Applicable Applications for anti-POLI antibody: ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: IHC: 1:20-1:200; IF: 1:50-1:200
• Immunogen: Recombinant Human DNA polymerase iota protein (458-711AA)
• Conjugation: Non-conjugated
• Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human testis tissue using MBS7103706 at dilution of 1:100)

Immunofluorescence (IF)

(Immunofluorescent analysis of Hela cells using MBS7103706 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-POLI antibody

Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.

Product Categories/Family for anti-POLI antibody

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 154350220
• NCBI GeneID: 11201
• NCBI Accession #: NP_009126.2
• NCBI GenBank Nucleotide #: NM_007195.2
• UniProt Accession #: Q9UNA4; Q8N590; Q9H0S1; Q9NYH6
• Molecular Weight: 83,006 Da
• NCBI Official Full Name: DNA polymerase iota isoform a (long)
• NCBI Official Synonym Full Names: DNA polymerase iota
• NCBI Official Symbol: POLI
• NCBI Official Synonym Symbols: eta2; RAD30B; RAD3OB
• NCBI Protein Information: DNA polymerase iota
• UniProt Protein Name: DNA polymerase iota
• Protein Family: Poliovirus receptor
• UniProt Gene Name: POLI
• UniProt Synonym Gene Names: RAD30B

NCBI Description

The protein encoded by this gene is an error-prone DNA polymerase involved in DNA repair. The encoded protein promotes DNA synthesis across lesions in the template DNA, which other polymerases cannot do. The encoded polymerase inserts deoxynucleotides across lesions and then relies on DNA polymerase zeta to extend the nascent DNA strand to bypass the lesion. [provided by RefSeq, May 2017]

Uniprot Description

Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.