IgM diluent
IgM-Reducing Assay Diluent
Synonyms
IgM; IgM-Reducing Assay Diluent; IgM diluent
Form/Format
Supplied at 1X.
Sequence Length
112
Application Notes
1. Once the capture antigen has been coated onto the plate and the plate has been properly blocked with one of ICT's stabilizing blocker products, pipette 50-100 uL of IgM-Reducing Assay Diluent into each of the wells of the ELISA plate using a multi-channel pipettor. The addition of the Assay Diluent is always done prior to adding the standard or test samples to each well.
2. The standard and test samples should be diluted to the proper well concentrations using one of ICT's three different Sample Diluent products to help further equalize matrix complexity parameters between standard/calibrator wells and the serum/plasma containing sample wells.
3. Because IgM-Reducing Assay Diluent contains a more diverse composition of additives, it can have some negative impact on the antigen capture step of the ELISA. This leads to reduction in ELISA sensitivity which must be compensated for by using longer antigen capture incubation times or more sensitive HRP substrate components.
2. The standard and test samples should be diluted to the proper well concentrations using one of ICT's three different Sample Diluent products to help further equalize matrix complexity parameters between standard/calibrator wells and the serum/plasma containing sample wells.
3. Because IgM-Reducing Assay Diluent contains a more diverse composition of additives, it can have some negative impact on the antigen capture step of the ELISA. This leads to reduction in ELISA sensitivity which must be compensated for by using longer antigen capture incubation times or more sensitive HRP substrate components.
pH
7.4 at 1X
Contains
Mammalian Proteins.
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for IgM diluent
Description: IgM-Reducing Assay Diluent is a specially formulated additive to address IgM-mediated conjugate bridging interference in problematic serum and plasma samples tested in antibody sandwich ELISA formats. IgM-Reducing Assay Diluent can be added to standard curve/calibrator and serum/plasma sample wells to help equalize matrix complexity disparity issues.
Background: IgM-Reducing Assay Diluent is an ELISA additive specially formulated for use with problematic serum and plasma samples tested in antibody sandwich ELISA formats. This diluent can be added to standard curve/calibrator and serum/plasma sample wells to help equalize matrix complexity disparity issues.
Large differences in matrix complexity environment between standard curve and sample wells are the main cause for target analyte under-recovery problems in sandwich ELISA analysis. Proprietary additives have been included in IgM-Reducing Assay Diluent to reduce IgM-mediated conjugate bridging interference that can otherwise cause substantial background noise.
IgM-Reducing Assay Diluent contains mammalian sera proteins to reduce nonspecific interactions that might occur between the sample matrix proteins and the plate surface. It also contains a proprietary calcium chelating agent designed to inhibit interference from complement and thrombin present in serum and plasma samples. An antimicrobial agent is included in the formulation to allow for bench-top use and storage stability.
Background: IgM-Reducing Assay Diluent is an ELISA additive specially formulated for use with problematic serum and plasma samples tested in antibody sandwich ELISA formats. This diluent can be added to standard curve/calibrator and serum/plasma sample wells to help equalize matrix complexity disparity issues.
Large differences in matrix complexity environment between standard curve and sample wells are the main cause for target analyte under-recovery problems in sandwich ELISA analysis. Proprietary additives have been included in IgM-Reducing Assay Diluent to reduce IgM-mediated conjugate bridging interference that can otherwise cause substantial background noise.
IgM-Reducing Assay Diluent contains mammalian sera proteins to reduce nonspecific interactions that might occur between the sample matrix proteins and the plate surface. It also contains a proprietary calcium chelating agent designed to inhibit interference from complement and thrombin present in serum and plasma samples. An antimicrobial agent is included in the formulation to allow for bench-top use and storage stability.
Product Categories/Family for IgM diluent
NCBI and Uniprot Product Information
Similar Products
Product Notes
The IgM (Catalog #AAA258386) is a Diluent and is intended for research purposes only. The product is available for immediate purchase. 1. Once the capture antigen has been coated onto the plate and the plate has been properly blocked with one of ICT's stabilizing blocker products, pipette 50-100 uL of IgM-Reducing Assay Diluent into each of the wells of the ELISA plate using a multi-channel pipettor. The addition of the Assay Diluent is always done prior to adding the standard or test samples to each well. 2. The standard and test samples should be diluted to the proper well concentrations using one of ICT's three different Sample Diluent products to help further equalize matrix complexity parameters between standard/calibrator wells and the serum/plasma containing sample wells. 3. Because IgM-Reducing Assay Diluent contains a more diverse composition of additives, it can have some negative impact on the antigen capture step of the ELISA. This leads to reduction in ELISA sensitivity which must be compensated for by using longer antigen capture incubation times or more sensitive HRP substrate components. Researchers should empirically determine the suitability of the IgM for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgM, Diluent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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