Western Blot (WB)
((0.3ug/ml) staining of human spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat PAD4/PADI4 Polyclonal Antibody | anti-PADI4 antibody
Goat anti-PAD4/PADI4 Antibody
Western Blot: Approx 74kDa band observed in Human Spleen and Liver lysates (calculated MW of 74.1kDa according to NP_036519.2). In transfected HEK293 transiently expressing PADI4 a band of approx.75kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1ug/ml.
Western Blot (WB)
((0.3ug/ml) staining of human spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of human spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
(HEK293 overexpressing PADI4 (RC206501) and probed (mock transfection in first lane), tested by Origene.)
Western Blot (WB)
(HEK293 overexpressing PADI4 (RC206501) and probed (mock transfection in first lane), tested by Origene.)
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of a gene family which encodes enzymes responsible for the conversion of arginine residues to citrulline residues. This gene may play a role in granulocyte and macrophage development leading to inflammation and immune response. [provided by RefSeq, Jul 2008]
Uniprot Description
PADI4: Catalyzes the citrullination/deimination of arginine residues of proteins. Citrullinates histone H3 at 'Arg-8' and/or 'Arg-17' and histone H4 at 'Arg-3', which prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1. Genetic variations in PADI4 are a cause of susceptibility to rheumatoid arthritis (RA). It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Could have an important role in the pathogenesis of rheumatoid arthritis by increasing citrullination of proteins in rheumatoid arthritis synovial tissues, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context. Belongs to the protein arginine deiminase family.
Protein type: Nuclear receptor co-regulator; EC 3.5.3.15; Hydrolase
Chromosomal Location of Human Ortholog: 1p36.13
Cellular Component: cytosol; nucleoplasm; nucleus
Molecular Function: arginine deiminase activity; calcium ion binding; protein binding; protein-arginine deiminase activity
Biological Process: chromatin modification; chromatin remodeling; establishment and/or maintenance of chromatin architecture; innate immune response; nucleosome assembly; peptidyl-citrulline biosynthetic process from peptidyl-arginine; protein modification process; regulation of transcription, DNA-dependent; stem cell maintenance; transcription, DNA-dependent
Disease: Rheumatoid Arthritis
Research Articles on PADI4
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Product Notes
The PADI4 padi4 (Catalog #AAA420131) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-PAD4/PADI4 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's PAD4/PADI4 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 64000. Western Blot: Approx 74kDa band observed in Human Spleen and Liver lysates (calculated MW of 74.1kDa according to NP_036519.2). In transfected HEK293 transiently expressing PADI4 a band of approx.75kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the PADI4 padi4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: AQGTLIRVTP EQPC. It is sometimes possible for the material contained within the vial of "PAD4/PADI4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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