Assay Diluent Optimization Pack: all four 100mL ADs Diluent
Assay Diluent Optimization Pack: all four 100mL ADs
Synonyms
Assay Diluent Optimization Pack: all four 100mL ADs; Assay Diluent Optimization Pack; Assay Diluent Optimization Pack: all four 100mL ADs diluent
Form/Format
Supplied at 1X.
Application Notes
1. To quickly determine which Assay Diluent formulation is best for the assay, all four Assay Diluents can be run in parallel on the same IgG-coated ELISA plate.
2. Determine the initial dynamic analyte detection range of the ELISA curves that were prepared in each of the four different Assay Diluent types.
3. Make test spikes into serum samples.
4. Read the spiked serum sample wells from each of the four AD-associated standard curves to see which Assay Diluent gives the best approximation of the spike concentration that was added to the serum samples.
5. In cases where a high level of matrix complexity interference is not detected, an additional Assay Diluent may not be required to obtain a useful standard curve and derived sample analysis.
2. Determine the initial dynamic analyte detection range of the ELISA curves that were prepared in each of the four different Assay Diluent types.
3. Make test spikes into serum samples.
4. Read the spiked serum sample wells from each of the four AD-associated standard curves to see which Assay Diluent gives the best approximation of the spike concentration that was added to the serum samples.
5. In cases where a high level of matrix complexity interference is not detected, an additional Assay Diluent may not be required to obtain a useful standard curve and derived sample analysis.
pH
7.4 at 1X
Contains
General Assay Diluent-Mammalian proteins; IgM-Reducing Assay Diluent-Mammalian proteins; Neptune Assay Diluent-Non-mammalian proteins; Antigen-Down Assay Diluent-Mammalian proteins.
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for Assay Diluent Optimization Pack: all four 100mL ADs diluent
Description: Assay Diluents are additive components used in an ELISA that equalize any differences between the sample matrices (serum, plasma, urine, cell culture fluid) and the calibrator diluent used to generate the standard curve of the ELISA. Assay Diluent Optimization Pack, containing four 100 mL ELISA development buffers (General Assay Diluent, IgM-Reducing Assay Diluent, Neptune Assay Diluent, and Antigen-Down Assay Diluent), offers an economical method of addressing matrix effects and optimizing signal-to-noise ratio in ELISA development projects.
Background: Achieving a high signal-to-noise ratio and a solid standard curve are goals of any ELISA development project, yet a number of complex issues may arise, presenting challenges to assay optimization. Many issues stem from differences between the sample and calibrator matrices and nonspecific binding of immunoassay components.
Most of the time invested in developing a new ELISA/EIA/RIA will involve selecting the proper calibrator matrix that matches most closely to the often very complex biological sample matrix that is being evaluated. Assay Diluents are additive components used in an ELISA to equalize any differences between the sample matrices (serum, plasma, urine, cell culture fluid) and the calibrator diluent used to generate the standard curve of the ELISA. We offer four ELISA development buffers that were designed to address these complex matrix effects and other assay concerns.
During ELISA development, it is often helpful to compare a number of different Assay Diluent formulations to better match the matrix complexity present in the sample wells with the standard curve calibrator matrix wells. Assay Diluent Optimization Pack, containing four ELISA development buffers (General Assay Diluent, IgM-Reducing Assay Diluent, Neptune Assay Diluent, and Antigen-Down Assay Diluent), offers an economical method of addressing matrix effects and optimizing signal-to-noise ratio in ELISA development projects.
Background: Achieving a high signal-to-noise ratio and a solid standard curve are goals of any ELISA development project, yet a number of complex issues may arise, presenting challenges to assay optimization. Many issues stem from differences between the sample and calibrator matrices and nonspecific binding of immunoassay components.
Most of the time invested in developing a new ELISA/EIA/RIA will involve selecting the proper calibrator matrix that matches most closely to the often very complex biological sample matrix that is being evaluated. Assay Diluents are additive components used in an ELISA to equalize any differences between the sample matrices (serum, plasma, urine, cell culture fluid) and the calibrator diluent used to generate the standard curve of the ELISA. We offer four ELISA development buffers that were designed to address these complex matrix effects and other assay concerns.
During ELISA development, it is often helpful to compare a number of different Assay Diluent formulations to better match the matrix complexity present in the sample wells with the standard curve calibrator matrix wells. Assay Diluent Optimization Pack, containing four ELISA development buffers (General Assay Diluent, IgM-Reducing Assay Diluent, Neptune Assay Diluent, and Antigen-Down Assay Diluent), offers an economical method of addressing matrix effects and optimizing signal-to-noise ratio in ELISA development projects.
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Product Notes
The Assay Diluent Optimization Pack: all four 100mL ADs (Catalog #AAA258063) is a Diluent and is intended for research purposes only. The product is available for immediate purchase. 1. To quickly determine which Assay Diluent formulation is best for the assay, all four Assay Diluents can be run in parallel on the same IgG-coated ELISA plate. 2. Determine the initial dynamic analyte detection range of the ELISA curves that were prepared in each of the four different Assay Diluent types. 3. Make test spikes into serum samples. 4. Read the spiked serum sample wells from each of the four AD-associated standard curves to see which Assay Diluent gives the best approximation of the spike concentration that was added to the serum samples. 5. In cases where a high level of matrix complexity interference is not detected, an additional Assay Diluent may not be required to obtain an useful standard curve and derived sample analysis. Researchers should empirically determine the suitability of the Assay Diluent Optimization Pack: all four 100mL ADs for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Assay Diluent Optimization Pack: all four 100mL ADs, Diluent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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